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Mendeley readers
Chapter title |
RNA Scaffold: Designed to Co-localize Enzymes
|
---|---|
Chapter number | 9 |
Book title |
RNA Scaffolds
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Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2730-2_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2729-6, 978-1-4939-2730-2
|
Authors |
ZJU_China Team (iGEM 2012), Ming Chen, Chen, Ming |
Abstract |
Self-assembling RNA scaffold is designed to co-localize enzymes in engineered biological pathways through interactions between scaffold's protein docking domains and their affinity protein-enzyme fusions, in vivo. Here we introduced a noncoding RNA structure theophylline aptamer that respond to theophylline ligand in order to modulate RNA scaffold and regulate multistep enzymatic activities. We described the specifically designed RNA scaffold increased the fluorescent intensity in a splitting GFP assay by 2.25-fold, and also observed a 1.43-fold increase in multi-enzymatic efficiency in IAA synthesis pathway. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
China | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 33% |
Unknown | 2 | 67% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 33% |
Unknown | 2 | 67% |