| Chapter title |
Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°.
|
|---|---|
| Chapter number | 15 |
| Book title |
RNA Scaffolds
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2730-2_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2729-6, 978-1-4939-2730-2
|
| Authors |
Khisamutdinov, Emil F, Bui, My Nguyen Hoan, Jasinski, Daniel, Zhao, Zhengyi, Cui, Zheng, Guo, Peixuan, Emil F. Khisamutdinov, My Nguyen Hoan Bui, Daniel Jasinski, Zhengyi Zhao, Zheng Cui, Peixuan Guo, Khisamutdinov, Emil F. |
| Abstract |
Precise shape control of architectures at the nanometer scale is an intriguing but extremely challenging facet. RNA has recently emerged as a unique material and thermostable building block for use in nanoparticle construction. Here, we describe a simple method from design to synthesis of RNA triangle, square, and pentagon by stretching RNA 3WJ native angle from 60° to 90° and 108°, using the three-way junction (3WJ) of the pRNA from bacteriophage phi29 dsDNA packaging motor. These methods for the construction of elegant polygons can be applied to other RNA building blocks including the utilization and application of RNA 4-way, 5-way, and other multi-way junctions. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 40% |
| Researcher | 2 | 20% |
| Student > Doctoral Student | 2 | 20% |
| Unknown | 2 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 20% |
| Chemistry | 2 | 20% |
| Unknown | 2 | 20% |