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RNA Scaffolds

Overview of attention for book
Cover of 'RNA Scaffolds'

Table of Contents

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    Book Overview
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    Chapter 1 A Method to Predict the 3D Structure of an RNA Scaffold
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    Chapter 2 Post-crystallization Improvement of RNA Crystal Diffraction Quality
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    Chapter 3 Expression and Purification of RNA–Protein Complexes in Escherichia coli
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    Chapter 4 Production of Homogeneous Recombinant RNA Using a tRNA Scaffold and Hammerhead Ribozymes
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    Chapter 5 In Vivo Production of Small Recombinant RNAs Embedded in a 5S rRNA-Derived Protective Scaffold
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    Chapter 6 Detection of RNA–Protein Interactions Using Tethered RNA Affinity Capture
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    Chapter 7 A Universal Method for Labeling Native RNA in Live Bacterial Cells
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    Chapter 8 Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors.
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    Chapter 9 RNA Scaffold: Designed to Co-localize Enzymes
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    Chapter 10 Artificial Ligase Ribozymes Isolated by a “Design and Selection” Strategy
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    Chapter 11 Engineering aptazyme switches for conditional gene expression in Mammalian cells utilizing an in vivo screening approach.
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    Chapter 12 Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells
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    Chapter 13 Design and Characterization of Topological Small RNAs.
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    Chapter 14 Folding RNA-Protein Complex into Designed Nanostructures.
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    Chapter 15 Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°.
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    Chapter 16 RNA-Mediated CdS-Based Nanostructures.
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    Chapter 17 An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA.
Attention for Chapter 17: An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA.
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Chapter title
An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA.
Chapter number 17
Book title
RNA Scaffolds
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2730-2_17
Pubmed ID
25967064
Book ISBNs
978-1-4939-2729-6, 978-1-4939-2730-2
Authors

Bak, Geunu, Choi, Jee Soo, Kim, Wonkyeong, Suk, Shinae, Lee, Younghoon, Geunu Bak, Jee Soo Choi, Wonkyeong Kim, Shinae Suk, Younghoon Lee

Abstract

Knockdown or silencing of a specific gene presents a powerful strategy for elucidating gene function in a variety of organisms. To date, efficient silencing methods have been established in eukaryotes, but not bacteria. In this chapter, an efficient and versatile gene silencing method using artificial small RNA (afsRNA) is described. For this purpose, target-recognizing sequences were introduced in specially designed RNA scaffolds to exist as single-stranded stretches in afsRNA. The translation initiation region of target genes was used as the sequence for afsRNA recognition, based on the theory that this site is usually highly accessible to ribosomes, and therefore, possibly, afsRNA.Two genes transcribed as monocistrons were tested with our protocol. Both genes were effectively silenced by their cognate afsRNAs.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 33%
Researcher 2 33%
Student > Master 1 17%
Unknown 1 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 33%
Biochemistry, Genetics and Molecular Biology 1 17%
Nursing and Health Professions 1 17%
Immunology and Microbiology 1 17%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2015.
All research outputs
#15,333,503
of 22,805,349 outputs
Outputs from Methods in molecular biology
#5,332
of 13,120 outputs
Outputs of similar age
#208,949
of 353,075 outputs
Outputs of similar age from Methods in molecular biology
#344
of 996 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,120 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,075 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 31st percentile – i.e., 31% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 996 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.

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