Chapter title |
Folding RNA-Protein Complex into Designed Nanostructures.
|
---|---|
Chapter number | 14 |
Book title |
RNA Scaffolds
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2730-2_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2729-6, 978-1-4939-2730-2
|
Authors |
Shibata, Tomonori, Suzuki, Yuki, Sugiyama, Hiroshi, Endo, Masayuki, Saito, Hirohide, Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Hirohide Saito |
Abstract |
RNA-protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution. |
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