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Mendeley readers
| Chapter title |
Expression and Purification of RNA–Protein Complexes in Escherichia coli
|
|---|---|
| Chapter number | 3 |
| Book title |
RNA Scaffolds
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2730-2_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2729-6, 978-1-4939-2730-2
|
| Authors |
Margueritte El Khouri, Marjorie Catala, Bili Seijo, Johana Chabal, Carine Tisné, Frédéric Dardel, Luc Ponchon, Khouri, Margueritte El, Catala, Marjorie, Seijo, Bili, Chabal, Johana, Tisné, Carine, Dardel, Frédéric, Ponchon, Luc |
| Abstract |
For structural, biochemical or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the "tRNA scaffold" method to RNA/protein co-expression in order to express and purify RNA by affinity in native condition. As a proof-of-concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 17% |
| Unknown | 5 | 83% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 50% |
| Other | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 50% |
| Agricultural and Biological Sciences | 1 | 17% |
| Unknown | 2 | 33% |