Chapter title |
Cre Recombinase: You Can’t Live with It, and You Can’t Live Without It
|
---|---|
Chapter number | 96 |
Book title |
Retinal Degenerative Diseases
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-17121-0_96 |
Pubmed ID | |
Book ISBNs |
978-3-31-917120-3, 978-3-31-917121-0
|
Authors |
Yun-Zheng Le, Meili Zhu, Robert E. Anderson, Le, Yun-Zheng, Zhu, Meili, Anderson, Robert E. |
Abstract |
The development of conditional gene targeting has greatly advanced our knowledge of human retinal diseases, but issues have arisen related to the use of some Cre-expressing mouse lines. In this article, we discuss potential problems associated with transgenic Cre expression-induced degeneration and alteration of rod photoreceptors and retinal pigment epithelium (RPE). Our strategy for circumventing RPE degeneration by induced transient Cre expression uses a single intravitreal doxycycline injection in a tetracycline-inducible RPE-specific Cre mouse line, which results in productive Cre-mediated recombination efficiently in the RPE. As constitutive expression of Cre in the RPE alters RPE biology, this inducible Cre/lox system provides an opportunity for conditional gene targeting in the RPE, a tissue that is closely related to photoreceptor degeneration, age-related macular degeneration, and diabetic retinopathy. |
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Geographical breakdown
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 25% |
Professor | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 3 | 38% |
Readers by discipline | Count | As % |
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Computer Science | 1 | 13% |
Medicine and Dentistry | 1 | 13% |
Neuroscience | 1 | 13% |
Other | 0 | 0% |
Unknown | 2 | 25% |