Chapter title |
Tamoxifen-Containing Eye Drops Successfully Trigger Cre -Mediated Recombination in the Entire Eye
|
---|---|
Chapter number | 66 |
Book title |
Retinal Degenerative Diseases
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-17121-0_66 |
Pubmed ID | |
Book ISBNs |
978-3-31-917120-3, 978-3-31-917121-0
|
Authors |
Anja Schlecht, Sarah V. Leimbeck, Ernst R. Tamm, Barbara M. Braunger, Schlecht, Anja, Leimbeck, Sarah V., Tamm, Ernst R., Braunger, Barbara M. |
Abstract |
Embryonic lethality in mice with targeted gene deletion is a major issue that can be circumvented by using Cre-loxP-based animal models. Various inducible Cre systems are available, e.g. such that are activated following tamoxifen treatment, and allow deletion of a specific target gene at any desired time point during the life span of the animal. In this study, we describe the efficiency of topical tamoxifen administration by eye drops using a Cre- reporter mouse strain (R26R). We report that tamoxifen-responsive CAGGCre-ER (TM) mice show a robust Cre- mediated recombination throughout the entire eye. |
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