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Glaucoma

Overview of attention for book
Cover of 'Glaucoma'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Use of Animal Models and Techniques in Glaucoma Research: Introduction
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    Chapter 2 Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
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    Chapter 3 The Microbead Occlusion Model of Ocular Hypertension in Mice
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    Chapter 4 Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods
  6. Altmetric Badge
    Chapter 5 Noninvasive Intraocular Pressure Measurement in Animals Models of Glaucoma
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    Chapter 6 High-Throughput Binocular Pattern Electroretinograms in the Mouse
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    Chapter 7 Visual Evoked Potentials in Glaucoma and Alzheimer’s Disease
  9. Altmetric Badge
    Chapter 8 Investigation of the Functional Retinal Output Using Microelectrode Arrays
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    Chapter 9 Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling
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    Chapter 10 Shotgun Sphingolipid Analysis of Human Aqueous Humor
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    Chapter 11 Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion
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    Chapter 12 Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models
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    Chapter 13 Quantification of Scleral Biomechanics and Collagen Fiber Alignment
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    Chapter 14 Biolistic Labeling of Retinal Ganglion Cells
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    Chapter 15 Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma
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    Chapter 16 In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration
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    Chapter 17 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues
  19. Altmetric Badge
    Chapter 18 Visualizing Astrocytes of the Optic Nerve
  20. Altmetric Badge
    Chapter 19 Investigation of MicroRNA Expression in Experimental Glaucoma
  21. Altmetric Badge
    Chapter 20 Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head
  22. Altmetric Badge
    Chapter 21 Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve
Attention for Chapter 21: Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve
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Chapter title
Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve
Chapter number 21
Book title
Glaucoma
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7407-8_21
Pubmed ID
Book ISBNs
978-1-4939-7406-1, 978-1-4939-7407-8
Authors

Hee Joo Choi, Rui Wang, Tatjana C. Jakobs

Abstract

Recent technological advances have extended the range of analytic tools to very small samples. It is now possible to assay the transcriptome, and in some cases even the proteome, of single cells reliably. This allows addressing novel questions, such as the genotype/phenotype relationships of single neurons, heterogeneity within individual cells of the same type, or the basis of differential vulnerability to injury. An important prerequisite for these kinds of studies is the ability to isolate well-defined individual cells without contamination by adjacent tissue. In the retina and optic nerve, cells of different types and functions are closely intermingled, limiting the use of standard methods such as laser capture microdissection. Here, we describe a simple method to isolate morphologically intact cells from the retina and the optic nerve and discuss considerations in recognizing and isolating different cell types after dissociation.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Unspecified 1 25%
Student > Doctoral Student 1 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 50%
Unspecified 1 25%
Neuroscience 1 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 December 2017.
All research outputs
#10,842,231
of 12,231,187 outputs
Outputs from Methods in molecular biology
#5,626
of 8,304 outputs
Outputs of similar age
#282,229
of 339,575 outputs
Outputs of similar age from Methods in molecular biology
#1,060
of 1,604 outputs
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