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Glaucoma

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Cover of 'Glaucoma'

Table of Contents

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    Book Overview
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    Chapter 1 Use of Animal Models and Techniques in Glaucoma Research: Introduction
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    Chapter 2 Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
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    Chapter 3 The Microbead Occlusion Model of Ocular Hypertension in Mice
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    Chapter 4 Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods
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    Chapter 5 Noninvasive Intraocular Pressure Measurement in Animals Models of Glaucoma
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    Chapter 6 High-Throughput Binocular Pattern Electroretinograms in the Mouse
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    Chapter 7 Visual Evoked Potentials in Glaucoma and Alzheimer’s Disease
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    Chapter 8 Investigation of the Functional Retinal Output Using Microelectrode Arrays
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    Chapter 9 Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling
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    Chapter 10 Shotgun Sphingolipid Analysis of Human Aqueous Humor
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    Chapter 11 Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion
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    Chapter 12 Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models
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    Chapter 13 Quantification of Scleral Biomechanics and Collagen Fiber Alignment
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    Chapter 14 Biolistic Labeling of Retinal Ganglion Cells
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    Chapter 15 Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma
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    Chapter 16 In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration
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    Chapter 17 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues
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    Chapter 18 Visualizing Astrocytes of the Optic Nerve
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    Chapter 19 Investigation of MicroRNA Expression in Experimental Glaucoma
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    Chapter 20 Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head
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    Chapter 21 Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve
Attention for Chapter 12: Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models
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Chapter title
Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models
Chapter number 12
Book title
Glaucoma
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7407-8_12
Pubmed ID
Book ISBNs
978-1-4939-7406-1, 978-1-4939-7407-8
Authors

Prabhavathi Maddineni, Ramesh B. Kasetti, Gulab S. Zode

Abstract

The pathological mechanisms underlying increased outflow resistance at the trabecular meshwork (TM) that is responsible for elevating intraocular pressure (IOP) have not been fully delineated. Recent studies have shown that progressive accumulation of misfolded proteins and induction of endoplasmic reticulum (ER) stress is associated with the pathophysiology of glaucomatous TM damage and IOP elevation. We have shown that known causes of human glaucoma, including expression of mutant myocilin or dexamethasone treatment induce abnormal protein accumulation and ER stress in the TM in vitro and in vivo models. To cope up with abnormal protein accumulation, TM cells activate a cytoprotective pathway of unfolded protein response (UPR). However, chronic ER stress can lead to TM dysfunction and IOP elevation. Using cell culture, mouse models, and human postmortem tissues as well as genetic and pharmacological manipulations, we have analyzed ER stress and UPR mediators in the glaucomatous TM damage and IOP elevation. In this chapter, we have described a detailed protocol for the analysis of protein misfolding and ER stress in TM cells and tissues and its association with glaucomatous TM damage and IOP elevation.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Professor 2 40%
Unspecified 1 20%
Student > Master 1 20%
Unknown 1 20%
Readers by discipline Count As %
Unspecified 1 20%
Nursing and Health Professions 1 20%
Agricultural and Biological Sciences 1 20%
Medicine and Dentistry 1 20%
Unknown 1 20%