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Clinical Applications of Mass Spectrometry in Biomolecular Analysis

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Cover of 'Clinical Applications of Mass Spectrometry in Biomolecular Analysis'

Table of Contents

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    Book Overview
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    Chapter 1 Mass Spectrometry in Clinical Laboratory: Applications in Biomolecular Analysis
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    Chapter 2 Quantification of Free Carnitine and Acylcarnitines in Plasma or Serum Using HPLC/MS/MS
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    Chapter 3 Quantification of Arginine and Its Methylated Derivatives in Plasma by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 4 Quantitation of Albumin in Urine by Liquid Chromatography Tandem Mass Spectrometry
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    Chapter 5 Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 6 Quantification of Five Clinically Important Amino Acids by HPLC-Triple TOF™ 5600 Based on Pre-column Double Derivatization Method
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    Chapter 7 Sensitive, Simple, and Robust Nano-Liquid Chromatography-Mass Spectrometry Method for Amyloid Protein Subtyping
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    Chapter 8 Quantitation of Ubiquinone (Coenzyme Q 10 ) in Serum/Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (ESI-LC-MS/MS)
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    Chapter 9 Quantitative Analysis of Salivary Cortisol Using LC-MS/MS
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    Chapter 10 Quantification of Dihydroxyacetone Phosphate (DHAP) in Human Red Blood Cells by HPLC-TripleTOF 5600™ Mass Spectrometer
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    Chapter 11 Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
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    Chapter 12 Direct Measurement of Free Estradiol in Human Serum and Plasma by Equilibrium Dialysis-Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 13 Quantification of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 14 Quantitation of Insulin Analogues in Serum Using Immunoaffinity Extraction, Liquid Chromatography, and Tandem Mass Spectrometry
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    Chapter 15 Quantitation of Insulin-Like Growth Factor 1 in Serum by Liquid Chromatography High Resolution Accurate-Mass Mass Spectrometry
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    Chapter 16 Quantitation of Free Metanephrines in Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 17 Quantification of Metanephrine and Normetanephrine in Urine Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 18 High-Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine by LC-MS/MS. Method for Analyzing Isomers Without Chromatographic Separation
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    Chapter 19 Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 20 Quantitative Organic Acids in Urine by Two Dimensional Gas Chromatography-Time of Flight Mass Spectrometry (GCxGC-TOFMS)
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    Chapter 21 High Sensitivity Measurement of Pancreatic Polypeptide and Its Variant in Serum and Plasma by LC-MS/MS
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    Chapter 22 Quantitation of Parathyroid Hormone in Serum or Plasma by Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 23 Determination of Phenylalanine and Tyrosine by High Performance Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 24 Urine Purine Metabolite Determination by UPLC-Tandem Mass Spectrometry.
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    Chapter 25 Urine Pyrimidine Metabolite Determination by HPLC Tandem Mass Spectrometry
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    Chapter 26 Quantitation of Plasma Renin Activity in Plasma Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
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    Chapter 27 Quantitation of S-Adenosylmethionine and S-Adenosylhomocysteine in Plasma Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry
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    Chapter 28 A Simple, High-Throughput Method for Analysis of Ceramide, Glucosylceramide, and Ceramide Trihexoside in Dried Blood Spots by LC/MS/MS
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    Chapter 29 Quantification of Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone by Liquid Chromatography-Tandem Mass Spectrometry (LC/MS/MS)
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    Chapter 30 Urinary Succinylacetone Analysis by Gas Chromatography-Mass Spectrometry (GC-MS)
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    Chapter 31 Quantification of 1,25-Dihydroxyvitamin D2 and D3 in Serum Using Liquid Chromatography-Tandem Mass Spectrometry
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    Chapter 32 High-Throughput Serum 25-Hydroxy Vitamin D Testing with Automated Sample Preparation
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    Chapter 33 Quantitation of 25-OH-Vitamin-D 2 and 25-OH-Vitamin-D 3 in Urine Using LC-MS/MS
Attention for Chapter 11: Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
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Chapter title
Simultaneous Quantitation of Estradiol and Estrone in Serum Using Liquid Chromatography Mass Spectrometry
Chapter number 11
Book title
Clinical Applications of Mass Spectrometry in Biomolecular Analysis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3182-8_11
Pubmed ID
Book ISBNs
978-1-4939-3181-1, 978-1-4939-3182-8
Authors

Catherine P. Riley, Richard E. Mathieu, Carmen Wiley

Abstract

Accurate measurement of the endogenous estrogens, estrone (E1) and estradiol (E2), is important in the clinical diagnosis and monitoring of multiple disorders. Typically, given the efficacy and low cost, radioimmunoassays (RIA) and enzyme-linked immunoassays (EIA) are used to quantify these hormones in biological samples. Unfortunately, at low levels these assays lack the necessary sensitivity and specificity for diagnosis of certain disorders in adult and pediatric endocrinology and oncology. In response to this need, we developed a fast and sensitive high performance liquid chromatography negative electrospray ionization tandem mass spectrometry (LC-MS/MS) method to measure serum estrone (E1) and estradiol (E2) without chemical derivatization. Samples are spiked with a stable isotopic carbon thirteen ((13)C) labeled internal standard and the estrogens are isolated by liquid-liquid extraction (LLE) with hexane:Methyl-tert-butyl ether (MTBE) (9:1). Following centrifugation and dry down samples are reconstituted with deionized water, and separated on a C18 reverse phase column. The analytes are quantified using a six point calibration curve with a linearity of 2.6-625 pg/ml and with a variability of less than 8 % across analytical range.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 25%
Other 1 13%
Student > Doctoral Student 1 13%
Lecturer 1 13%
Student > Bachelor 1 13%
Other 1 13%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Environmental Science 1 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 13%
Medicine and Dentistry 1 13%
Unknown 2 25%