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Biotechnology of Plant Secondary Metabolism

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Cover of 'Biotechnology of Plant Secondary Metabolism'

Table of Contents

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    Book Overview
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    Chapter 1 Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal.
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    Chapter 2 Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
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    Chapter 3 A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
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    Chapter 4 Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
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    Chapter 5 From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
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    Chapter 6 Production of Recombinant Caffeine Synthase from Guarana ( Paullinia cupana var. sorbilis ) in Escherichia coli
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    Chapter 7 Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
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    Chapter 8 Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
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    Chapter 9 Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
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    Chapter 10 A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
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    Chapter 11 Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
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    Chapter 12 Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
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    Chapter 13 Protoplast Transformation as a Plant-Transferable Transient Expression System
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    Chapter 14 Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System.
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    Chapter 15 Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
Attention for Chapter 3: A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
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Chapter title
A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
Chapter number 3
Book title
Biotechnology of Plant Secondary Metabolism
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3393-8_3
Pubmed ID
Book ISBNs
978-1-4939-3391-4, 978-1-4939-3393-8
Authors

Júlio César de Lima, Thanise Nogueira Füller, Fernanda de Costa, Kelly C. S. Rodrigues-Corrêa, Arthur G. Fett-Neto

Abstract

RNA extraction resulting in good yields and quality is a fundamental step for the analyses of transcriptomes through high-throughput sequencing technologies, microarray, and also northern blots, RT-PCR, and RTqPCR. Even though many specific protocols designed for plants with high content of secondary metabolites have been developed, these are often expensive, time consuming, and not suitable for a wide range of tissues. Here we present a modification of the method previously described using the commercially available Concert™ Plant RNA Reagent (Invitrogen) buffer for field-grown adult pine trees with high oleoresin content.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 40%
Student > Bachelor 1 20%
Researcher 1 20%
Unknown 1 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 60%
Immunology and Microbiology 1 20%
Unknown 1 20%