Chapter title |
A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
|
---|---|
Chapter number | 3 |
Book title |
Biotechnology of Plant Secondary Metabolism
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3393-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3391-4, 978-1-4939-3393-8
|
Authors |
Júlio César de Lima, Thanise Nogueira Füller, Fernanda de Costa, Kelly C. S. Rodrigues-Corrêa, Arthur G. Fett-Neto, de Lima, Júlio César, Füller, Thanise Nogueira, de Costa, Fernanda, Rodrigues-Corrêa, Kelly C. S., Fett-Neto, Arthur G. |
Abstract |
RNA extraction resulting in good yields and quality is a fundamental step for the analyses of transcriptomes through high-throughput sequencing technologies, microarray, and also northern blots, RT-PCR, and RTqPCR. Even though many specific protocols designed for plants with high content of secondary metabolites have been developed, these are often expensive, time consuming, and not suitable for a wide range of tissues. Here we present a modification of the method previously described using the commercially available Concert™ Plant RNA Reagent (Invitrogen) buffer for field-grown adult pine trees with high oleoresin content. |
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