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Mendeley readers
| Chapter title |
Protoplast Transformation as a Plant-Transferable Transient Expression System
|
|---|---|
| Chapter number | 13 |
| Book title |
Biotechnology of Plant Secondary Metabolism
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3393-8_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3391-4, 978-1-4939-3393-8
|
| Authors |
Patrícia Duarte, Diana Ribeiro, Inês Carqueijeiro, Sara Bettencourt, Mariana Sottomayor, Duarte, Patrícia, Ribeiro, Diana, Carqueijeiro, Inês, Bettencourt, Sara, Sottomayor, Mariana |
| Abstract |
The direct uptake of DNA by naked plant cells (protoplasts) provides an expression system of exception for the quickly growing research in non-model plants, fuelled by the power of next-generation sequencing to identify novel candidate genes. Here, we describe a simple and effective method for isolation and transformation of protoplasts, and illustrate its application to several plant materials. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 20% |
| Student > Ph. D. Student | 3 | 15% |
| Student > Master | 2 | 10% |
| Student > Doctoral Student | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 3 | 15% |
| Unknown | 6 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 35% |
| Biochemistry, Genetics and Molecular Biology | 5 | 25% |
| Environmental Science | 1 | 5% |
| Engineering | 1 | 5% |
| Unknown | 6 | 30% |