↓ Skip to main content

Biotechnology of Plant Secondary Metabolism

Overview of attention for book
Cover of 'Biotechnology of Plant Secondary Metabolism'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal.
  3. Altmetric Badge
    Chapter 2 Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
  4. Altmetric Badge
    Chapter 3 A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
  5. Altmetric Badge
    Chapter 4 Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
  6. Altmetric Badge
    Chapter 5 From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
  7. Altmetric Badge
    Chapter 6 Production of Recombinant Caffeine Synthase from Guarana ( Paullinia cupana var. sorbilis ) in Escherichia coli
  8. Altmetric Badge
    Chapter 7 Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
  9. Altmetric Badge
    Chapter 8 Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast.
  10. Altmetric Badge
    Chapter 9 Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
  11. Altmetric Badge
    Chapter 10 A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
  12. Altmetric Badge
    Chapter 11 Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
  13. Altmetric Badge
    Chapter 12 Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
  14. Altmetric Badge
    Chapter 13 Protoplast Transformation as a Plant-Transferable Transient Expression System
  15. Altmetric Badge
    Chapter 14 Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System.
  16. Altmetric Badge
    Chapter 15 Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs.
Attention for Chapter 6: Production of Recombinant Caffeine Synthase from Guarana ( Paullinia cupana var. sorbilis ) in Escherichia coli
Altmetric Badge

Citations

dimensions_citation
1 Dimensions

Readers on

mendeley
20 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Production of Recombinant Caffeine Synthase from Guarana ( Paullinia cupana var. sorbilis ) in Escherichia coli
Chapter number 6
Book title
Biotechnology of Plant Secondary Metabolism
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3393-8_6
Pubmed ID
Book ISBNs
978-1-4939-3391-4, 978-1-4939-3393-8
Authors

Flávia Camila Schimpl, Eduardo Kiyota, Paulo Mazzafera

Abstract

Caffeine synthase (CS) is a methyltransferase responsible for the last two steps of the caffeine biosynthesis pathway in plants. CS is able to convert 7-methylxanthine to theobromine (3,7-dimethylxanthine) and theobromine to caffeine (1,3,7-trimethylxanthine) using S-adenosyl-L-methionine as the methyl donor in both reactions. The production of a recombinant protein is an important tool for the characterization of enzymes, particularly when the enzyme has affinity for different substrates. Guarana has the highest caffeine content among more than a hundred plant species that contain this alkaloid. Different from other plants, in which CS has a higher affinity for paraxanthine (1,7-dimethylxanthine), caffeine synthase from guarana (PcCS) has a higher affinity for theobromine. Here, we describe a method to produce a recombinant caffeine synthase from guarana in Escherichia coli and its purification by affinity chromatography. The recombinant protein retains activity and can be used in enzymatic assays and other biochemical characterization studies.

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Professor 5 25%
Student > Bachelor 3 15%
Student > Ph. D. Student 2 10%
Student > Master 2 10%
Other 1 5%
Other 2 10%
Unknown 5 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 35%
Biochemistry, Genetics and Molecular Biology 4 20%
Psychology 1 5%
Immunology and Microbiology 1 5%
Unknown 7 35%