| Chapter title |
Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks
|
|---|---|
| Chapter number | 14 |
| Book title |
RNA-Protein Complexes and Interactions
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3591-8_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3589-5, 978-1-4939-3591-8
|
| Authors |
Hsin-Sung Yeh, Jae-Woong Chang, Jeongsik Yong, Yeh, Hsin-Sung, Chang, Jae-Woong, Yong, Jeongsik |
| Abstract |
Characterizing protein-protein and protein-RNA interaction networks is a fundamental step to understanding the function of an RNA-binding protein. In many cases, these interactions are transient and highly dynamic. Therefore, capturing stable as well as transient interactions in living cells for the identification of protein-binding partners and the mapping of RNA-binding sequences is key to a successful establishment of the molecular interaction network. In this chapter, we will describe a method for capturing the molecular interactions in living cells using formaldehyde as a crosslinker and enriching a specific RNA-protein complex from cell extracts followed by mass spectrometry and Next-Gen sequencing analyses. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 44% |
| Professor > Associate Professor | 1 | 11% |
| Other | 1 | 11% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 33% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Computer Science | 1 | 11% |
| Neuroscience | 1 | 11% |
| Unknown | 3 | 33% |