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Preface.

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Cover of 'Preface.'

Table of Contents

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    Book Overview
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    Chapter 1 RNA-Protein Complexes and Interactions
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    Chapter 2 Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)
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    Chapter 3 Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro
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    Chapter 4 Detecting RNA–Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting
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    Chapter 5 Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture
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    Chapter 6 Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
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    Chapter 7 RNA-Protein Complexes and Interactions
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    Chapter 8 Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases
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    Chapter 9 RNA-Protein Complexes and Interactions
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    Chapter 10 Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixture
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    Chapter 11 RNA-Protein Complexes and Interactions
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    Chapter 12 RNA-Protein Complexes and Interactions
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    Chapter 13 RNA-Protein Complexes and Interactions
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    Chapter 14 Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks
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    Chapter 15 RNA-Protein Complexes and Interactions
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    Chapter 16 Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
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    Chapter 17 RNA-Protein Complexes and Interactions
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    Chapter 18 RNA-Protein Complexes and Interactions
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    Chapter 19 Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5′ Splice-Site Base-Pairing
  21. Altmetric Badge
    Chapter 20 Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter
Attention for Chapter 1: RNA-Protein Complexes and Interactions
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Chapter title
RNA-Protein Complexes and Interactions
Chapter number 1
Book title
RNA-Protein Complexes and Interactions
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3591-8_1
Pubmed ID
Book ISBNs
978-1-4939-3589-5, 978-1-4939-3591-8
Authors

Skrdlant, Lindsey, Lin, Ren-Jang, Lindsey Skrdlant, Ren-Jang Lin

Abstract

SR proteins are a class of RNA-binding proteins whose RNA-binding ability is required for both constitutive and alternative splicing. While members of the SR protein family were once thought to have redundant functions, in-depth biochemical analysis of their RNA-binding abilities has revealed distinct binding profiles for each SR protein, that often lead to either synergistic or antagonistic functions. SR protein family members SRSF1 and SRSF2 are two of the most highly studied RNA-binding proteins. Here we examine the various methods used to differentiate SRSF1 and SRSF2 RNA-binding ability. We discuss the benefits and type of information that can be determined using each method.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 4%
China 1 4%
Unknown 21 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 22%
Student > Doctoral Student 3 13%
Researcher 3 13%
Professor 3 13%
Student > Bachelor 2 9%
Other 2 9%
Unknown 5 22%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 43%
Agricultural and Biological Sciences 6 26%
Chemical Engineering 1 4%
Neuroscience 1 4%
Unknown 5 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 March 2016.
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#20,315,221
of 22,856,968 outputs
Outputs from Methods in molecular biology
#9,917
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#330,662
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Outputs of similar age from Methods in molecular biology
#1,053
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