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Preface.

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Cover of 'Preface.'

Table of Contents

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    Book Overview
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    Chapter 1 RNA-Protein Complexes and Interactions
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    Chapter 2 Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification)
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    Chapter 3 Biotin–Streptavidin Affinity Purification of RNA–Protein Complexes Assembled In Vitro
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    Chapter 4 Detecting RNA–Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting
  6. Altmetric Badge
    Chapter 5 Purification of RNA–Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture
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    Chapter 6 Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts
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    Chapter 7 RNA-Protein Complexes and Interactions
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    Chapter 8 Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases
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    Chapter 9 RNA-Protein Complexes and Interactions
  11. Altmetric Badge
    Chapter 10 Northwestern Blot Analysis: Detecting RNA–Protein Interaction After Gel Separation of Protein Mixture
  12. Altmetric Badge
    Chapter 11 RNA-Protein Complexes and Interactions
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    Chapter 12 RNA-Protein Complexes and Interactions
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    Chapter 13 RNA-Protein Complexes and Interactions
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    Chapter 14 Ribo-Proteomics Approach to Profile RNA–Protein and Protein–Protein Interaction Networks
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    Chapter 15 RNA-Protein Complexes and Interactions
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    Chapter 16 Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX
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    Chapter 17 RNA-Protein Complexes and Interactions
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    Chapter 18 RNA-Protein Complexes and Interactions
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    Chapter 19 Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5′ Splice-Site Base-Pairing
  21. Altmetric Badge
    Chapter 20 Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter
Attention for Chapter 11: RNA-Protein Complexes and Interactions
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Chapter title
RNA-Protein Complexes and Interactions
Chapter number 11
Book title
RNA-Protein Complexes and Interactions
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3591-8_11
Pubmed ID
Book ISBNs
978-1-4939-3589-5, 978-1-4939-3591-8
Authors

Kudla, Marek, Karginov, Fedor V, Marek Kudla, Fedor V. Karginov, Karginov, Fedor V.

Abstract

Determination of mRNA translation rates is essential to understanding the regulatory pathways governing eukaryotic gene expression. In this chapter, we present a transcriptome-wide method to assess translation by association of mRNAs with polysomes on sucrose density gradients. After sedimentation, the fractions are spiked with a control RNA mixture and the RNA content is measured by high-throughput sequencing. Normalization to the spike-ins provides a global quantitative view on the translational status of cellular mRNAs, with the ability to measure changes and identify active and silent subpopulations of each.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 31%
Professor 2 13%
Other 2 13%
Student > Master 1 6%
Researcher 1 6%
Other 0 0%
Unknown 5 31%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 38%
Agricultural and Biological Sciences 3 19%
Neuroscience 1 6%
Unknown 6 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 March 2016.
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#15,364,458
of 22,856,968 outputs
Outputs from Methods in molecular biology
#5,349
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#230,906
of 393,628 outputs
Outputs of similar age from Methods in molecular biology
#545
of 1,470 outputs
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