| Chapter title |
Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
|
|---|---|
| Chapter number | 11 |
| Book title |
Biotechnology of Plant Secondary Metabolism
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3393-8_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3391-4, 978-1-4939-3393-8
|
| Authors |
Márcia R. de Almeida, Martina V. Strömvik, de Almeida, Márcia R., Strömvik, Martina V. |
| Abstract |
Laser capture microdissection (LCM) is a powerful technique for harvesting specific cells from a heterogeneous population. As each cell and tissue has its unique genetic, proteomic, and metabolic profile, the use of homogeneous samples is important for a better understanding of complex processes in both animal and plant systems. In case of plants, LCM is very suitable as the highly regular tissue organization and stable cell walls from these organisms enable visual identification of various cell types without staining of tissue sections, which can prevent some downstream analysis. Considering the applicability of LCM to any plant species, here we provide a step-by-step protocol for selecting specific cells or tissues through this technology. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 50% |
| Student > Ph. D. Student | 1 | 25% |
| Unknown | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Unknown | 2 | 50% |