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Nanoscale Imaging

Overview of attention for book
Cover of 'Nanoscale Imaging'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 High-Resolution Atomic Force Microscopy Imaging of Nucleic Acids
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    Chapter 2 Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing
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    Chapter 3 AFM-Based Single-Molecule Force Spectroscopy of Proteins
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    Chapter 4 High-Resolution AFM-Based Force Spectroscopy
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    Chapter 5 Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions
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    Chapter 6 Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers
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    Chapter 7 AFM-Based Characterization of Electrical Properties of Materials
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    Chapter 8 Supported Lipid Bilayers for Atomic Force Microscopy Studies
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    Chapter 9 Quantifying Small Molecule Binding Interactions with DNA Nanostructures
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    Chapter 10 Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy
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    Chapter 11 High-Resolution and High-Speed Atomic Force Microscope Imaging
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    Chapter 12 High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies
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    Chapter 13 Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged Using High-Speed AFM
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    Chapter 14 Assembly of Centromere Chromatin for Characterization by High-Speed Time-Lapse Atomic Force Microscopy
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    Chapter 15 High-Speed Force Spectroscopy for Single Protein Unfolding
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    Chapter 16 Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assays
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    Chapter 17 Preparing Frozen-Hydrated Protein–Nucleic Acid Assemblies for High-Resolution Cryo-EM Imaging
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    Chapter 18 Probing Chromatin Structure with Magnetic Tweezers
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    Chapter 19 Single-Molecule and Ensemble Methods to Probe Initial Stages of RNP Granule Assembly
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    Chapter 20 Correlative Atomic Force and Single-Molecule Fluorescence Microscopy of Nucleoprotein Complexes
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    Chapter 21 Sensing the Ultrastructure of Bacterial Surfaces and Their Molecular Binding Forces Using AFM
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    Chapter 22 Nanoscale Visualization of Bacterial Microcompartments Using Atomic Force Microscopy
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    Chapter 23 Time-Resolved Imaging of Bacterial Surfaces Using Atomic Force Microscopy
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    Chapter 24 Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy
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    Chapter 25 Fluorescence Correlation Spectroscopy on Genomic DNA in Living Cells
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    Chapter 26 Three-Dimensional Tracking of Quantum Dot-Conjugated Molecules in Living Cells
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    Chapter 27 AFM Indentation Analysis of Cells to Study Cell Mechanics and Pericellular Coat
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    Chapter 28 Imaging of Soft and Biological Samples Using AFM Ringing Mode
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    Chapter 29 Probing Single Virus Binding Sites on Living Mammalian Cells Using AFM
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    Chapter 30 Applications of Atomic Force Microscopy for Adhesion Force Measurements in Mechanotransduction
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    Chapter 31 Methods for Atomic Force Microscopy of Biological and Living Specimens
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    Chapter 32 Single Molecule Imaging in Live Embryos Using Lattice Light-Sheet Microscopy
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    Chapter 33 Silver Filler Pre-embedding to Enhance Resolution and Contrast in Multidimensional SEM: A Nanoscale Imaging Study on Liver Tissue
  35. Altmetric Badge
    Chapter 34 Nanoscale Dynamics and Energetics of Proteins and Protein-Nucleic Acid Complexes in Classical Molecular Dynamics Simulations
Attention for Chapter 15: High-Speed Force Spectroscopy for Single Protein Unfolding
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Chapter title
High-Speed Force Spectroscopy for Single Protein Unfolding
Chapter number 15
Book title
Nanoscale Imaging
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-8591-3_15
Pubmed ID
Book ISBNs
978-1-4939-8590-6, 978-1-4939-8591-3
Authors

Fidan Sumbul, Arin Marchesi, Hirohide Takahashi, Simon Scheuring, Felix Rico

Abstract

Single-molecule force spectroscopy (SMFS) measurements allow for quantification of the molecular forces required to unfold individual protein domains. Atomic force microscopy (AFM) is one of the long-established techniques for force spectroscopy (FS). Although FS at conventional AFM pulling rates provides valuable information on protein unfolding, in order to get a more complete picture of the mechanism, explore new regimes, and combine and compare experiments with simulations, we need higher pulling rates and μs-time resolution, now accessible via high-speed force spectroscopy (HS-FS). In this chapter, we provide a step-by-step protocol of HS-FS including sample preparation, measurements and analysis of the acquired data using HS-AFM with an illustrative example on unfolding of a well-studied concatamer made of eight repeats of the titin I91 domain.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 18%
Student > Doctoral Student 2 18%
Student > Master 2 18%
Researcher 1 9%
Unknown 4 36%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 18%
Physics and Astronomy 1 9%
Chemical Engineering 1 9%
Biochemistry, Genetics and Molecular Biology 1 9%
Social Sciences 1 9%
Other 2 18%
Unknown 3 27%

Attention Score in Context

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#6,290
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