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Xenopus

Overview of attention for book
Cover of 'Xenopus'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Husbandry, General Care, and Transportation of Xenopus laevis and Xenopus tropicalis
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    Chapter 2 Generation and Care of Xenopus laevis and Xenopus tropicalis Embryos
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    Chapter 3 Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering
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    Chapter 4 Targeted Genome Engineering in Xenopus Using the Transcription Activator-Like Effector Nuclease (TALEN) Technology
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    Chapter 5 Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis
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    Chapter 6 BATCH-GE: Analysis of NGS Data for Genome Editing Assessment
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    Chapter 7 A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair
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    Chapter 8 How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations in F0 Xenopus Through the Host-Transfer Technique
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    Chapter 9 Targeted Electroporation in the CNS in Xenopus Embryos
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    Chapter 10 Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus Retina
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    Chapter 11 Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors
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    Chapter 12 CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis
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    Chapter 13 Quantitative Proteomics of Xenopus Embryos I, Sample Preparation
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    Chapter 14 Quantitative Proteomics for Xenopus Embryos II, Data Analysis
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    Chapter 15 Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous System
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    Chapter 16 X-FaCT: Xenopus-Fast Clearing Technique
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    Chapter 17 Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry
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    Chapter 18 Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary Epidermis
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    Chapter 19 Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin
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    Chapter 20 Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow Cells
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    Chapter 21 Isolation and Primary Culture Methods of Adult and Larval Myogenic Cells from Xenopus laevis
Attention for Chapter 11: Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors
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Chapter title
Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors
Chapter number 11
Book title
Xenopus
Published in
Methods in molecular biology, August 2018
DOI 10.1007/978-1-4939-8784-9_11
Pubmed ID
Book ISBNs
978-1-4939-8783-2, 978-1-4939-8784-9
Authors

Thomas Naert, Kris Vleminckx

Abstract

The recent advent of CRISPR/Cas9 as a straightforward genome editing tool has allowed the establishment of the first bona fide genetic cancer models within the diploid aquatic model organism Xenopus tropicalis (X. tropicalis). Within this chapter, we demonstrate the methods for targeting tumor suppressors with the CRISPR/Cas9 system in the developing X. tropicalis embryo. We further illustrate genotyping and phenotyping of the resulting tumor-bearing F0 mosaic mutant animals (crispants). We focus in detail on the histopathological analysis of cancer neoplasms, the methodology to illustrate high proliferative index by proliferation marker immunofluorescence and how to isolate specific (tumor) cell populations by laser capture microdissection. As such, the described pipeline allows for rapid establishment of novel cancer models by CRISPR/Cas9 targeting of established tumor suppressor genes, or novel candidates obtained from clinical data. In conclusion, we thus provide the methodology for modeling human cancer with the highly efficient CRISPR/Cas9 system in F0 X. tropicalis.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 50%
Researcher 2 25%
Professor 1 13%
Unknown 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 38%
Agricultural and Biological Sciences 2 25%
Chemical Engineering 1 13%
Unknown 2 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2018.
All research outputs
#11,933,637
of 13,457,774 outputs
Outputs from Methods in molecular biology
#5,974
of 8,487 outputs
Outputs of similar age
#229,792
of 266,463 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
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