Chapter title |
Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses
|
---|---|
Chapter number | 11 |
Book title |
Vibrio Cholerae
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8685-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8684-2, 978-1-4939-8685-9
|
Authors |
Paul Kohl, Franz G. Zingl, Thomas O. Eichmann, Stefan Schild, Kohl, Paul, Zingl, Franz G., Eichmann, Thomas O., Schild, Stefan |
Abstract |
Outer membrane vesicles (OMVs) are naturally secreted from the bacterial cell surface and therefore localized in the cell-free supernatant of bacterial cultures. Here we describe methods for crude and density gradient-purified OMV isolation and protocols for control analyses for protein profiling (SDS-PAGE), detection of indicator proteins (immunoblot analysis), lipid profiling (lipid extraction and LC-MS analysis), vesicle size determination (NanoSight), rough estimation of biomass (TrayCell™), as well as quantifications of defined OMV components, e.g., proteins (Bradford) and LPS (Purpald). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 45 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 6 | 13% |
Student > Ph. D. Student | 6 | 13% |
Researcher | 6 | 13% |
Student > Master | 6 | 13% |
Student > Doctoral Student | 4 | 9% |
Other | 5 | 11% |
Unknown | 12 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 15 | 33% |
Agricultural and Biological Sciences | 6 | 13% |
Immunology and Microbiology | 4 | 9% |
Engineering | 2 | 4% |
Veterinary Science and Veterinary Medicine | 1 | 2% |
Other | 3 | 7% |
Unknown | 14 | 31% |