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Capillary Electrophoresis

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Cover of 'Capillary Electrophoresis'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 The CE-Way of Thinking: "All Is Relative!".
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    Chapter 2 A Semiempirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis.
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    Chapter 3 Derivatization in Capillary Electrophoresis.
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    Chapter 4 Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis: Strategies and Implementation.
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    Chapter 5 Micellar Electrokinetic Chromatography of Aminoglycosides.
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    Chapter 6 Microemulsion Electrokinetic Chromatography.
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    Chapter 7 Nonaqueous Capillary Electrophoresis Mass Spectrometry.
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    Chapter 8 Ionic Liquids in Capillary Electrophoresis.
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    Chapter 9 CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.
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    Chapter 10 Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry.
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    Chapter 11 Use of CE to Analyze Solutes in Pico- and Nano-Liter Samples from Plant Cells and Rhizosphere.
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    Chapter 12 Analysis of Small Ions with Capillary Electrophoresis.
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    Chapter 13 Metal Ions Analysis with Capillary Zone Electrophoresis.
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    Chapter 14 Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis.
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    Chapter 15 Enantiomer Separations by Capillary Electrophoresis.
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    Chapter 16 Capillary Electrophoresis of Mono- and Oligosaccharides.
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    Chapter 17 Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.
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    Chapter 18 Separation of Peptides by Capillary Electrophoresis.
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    Chapter 19 Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach.
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    Chapter 20 Capillary Electrophoretic Analysis of Classical Organic Pollutants.
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    Chapter 21 Capillary Electrophoresis in Metabolomics.
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    Chapter 22 Capillary Electrophoresis in Food and Foodomics.
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    Chapter 23 Capillary Electrophoresis in Wine Science.
Attention for Chapter 4: Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis: Strategies and Implementation.
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Chapter title
Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis: Strategies and Implementation.
Chapter number 4
Book title
Capillary Electrophoresis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6403-1_4
Pubmed ID
Book ISBNs
978-1-4939-6401-7, 978-1-4939-6403-1
Authors

Martin Pattky, Katalin Barkovits, Katrin Marcus, Oliver H. Weiergräber, Carolin Huhn

Editors

Philippe Schmitt-Kopplin

Abstract

Coatings are necessary to prevent protein and peptide adsorption to the capillary surface and obtain high intermediate precision. In this protocol, we first present our basic strategy to address peptide separation using three different coatings: one neutral and two cationic coatings, the latter largely differing in their induced electroosmotic mobility. In detail, we will describe how we apply the statically adsorbed coatings to obtain very high plate numbers and high repeatability.With some model examples, we clearly describe the scope of the method for the analysis of peptide samples: tryptic digests are addressed as well as small glycoproteins and glycopeptides largely differing in their effective electrophoretic mobility. We also show that the method is suitable for a fast screening of peptide samples despite a high matrix load comprising of up to 500 mmol/L sodium chloride. We demonstrate that this basic CE-MS method is rather independent of the polarity of the analytes with a very fast near-baseline separation of very hydrophobic Aβ peptides related to the onset of Alzheimer's disease. These examples will give an impression, which coating is most suitable for a specific analytical application.Special attention is paid to difficult aspects of the coating procedure and the CE-MS method, e.g., the potential of cross-contamination when changing the coatings.

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Readers by professional status Count As %
Student > Ph. D. Student 1 100%
Readers by discipline Count As %
Business, Management and Accounting 1 100%