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Capillary Electrophoresis

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Cover of 'Capillary Electrophoresis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The CE-Way of Thinking: "All Is Relative!".
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    Chapter 2 A Semiempirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis.
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    Chapter 3 Derivatization in Capillary Electrophoresis.
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    Chapter 4 Statically Adsorbed Coatings for High Separation Efficiency and Resolution in CE-MS Peptide Analysis: Strategies and Implementation.
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    Chapter 5 Micellar Electrokinetic Chromatography of Aminoglycosides.
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    Chapter 6 Microemulsion Electrokinetic Chromatography.
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    Chapter 7 Nonaqueous Capillary Electrophoresis Mass Spectrometry.
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    Chapter 8 Ionic Liquids in Capillary Electrophoresis.
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    Chapter 9 CZE-CZE ESI-MS Coupling with a Fully Isolated Mechanical Valve.
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    Chapter 10 Capillary Electrophoresis-Inductively Coupled Plasma Mass Spectrometry.
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    Chapter 11 Use of CE to Analyze Solutes in Pico- and Nano-Liter Samples from Plant Cells and Rhizosphere.
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    Chapter 12 Analysis of Small Ions with Capillary Electrophoresis.
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    Chapter 13 Metal Ions Analysis with Capillary Zone Electrophoresis.
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    Chapter 14 Bioanalytical Application of Amino Acid Detection by Capillary Electrophoresis.
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    Chapter 15 Enantiomer Separations by Capillary Electrophoresis.
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    Chapter 16 Capillary Electrophoresis of Mono- and Oligosaccharides.
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    Chapter 17 Use of Capillary Electrophoresis for Polysaccharide Studies and Applications.
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    Chapter 18 Separation of Peptides by Capillary Electrophoresis.
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    Chapter 19 Microbial Analysis of Escherichia coli ATCC, Lactobacteria and Saccharomyces cerevisiae Using Capillary Electrophoresis Approach.
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    Chapter 20 Capillary Electrophoretic Analysis of Classical Organic Pollutants.
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    Chapter 21 Capillary Electrophoresis in Metabolomics.
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    Chapter 22 Capillary Electrophoresis in Food and Foodomics.
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    Chapter 23 Capillary Electrophoresis in Wine Science.
Attention for Chapter 3: Derivatization in Capillary Electrophoresis.
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Chapter title
Derivatization in Capillary Electrophoresis.
Chapter number 3
Book title
Capillary Electrophoresis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6403-1_3
Pubmed ID
Book ISBNs
978-1-4939-6401-7, 978-1-4939-6403-1
Authors

M. Luisa Marina, María Castro-Puyana

Editors

Philippe Schmitt-Kopplin

Abstract

Capillary electrophoresis is a well-established separation technique in analytical research laboratories worldwide. Its interesting advantages make CE an efficient and potent alternative to other chromatographic techniques. However, it is also recognized that its main drawback is the relatively poor sensitivity when using optical detection. One way to overcome this limitation is to perform a derivatization reaction which is intended to provide the analyte more suitable analytical characteristics enabling a high sensitive detection. Based on the analytical step where the CE derivatization takes place, it can be classified as precapillary (before separation), in-capillary (during separation), or postcapillary (after separation). This chapter describes the application of four different derivatization protocols (in-capillary and precapillary modes) to carry out the achiral and chiral analysis of different compounds in food and biological samples with three different detection modes (UV, LIF, and MS).

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 3 9%
Unknown 32 91%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 31%
Researcher 6 17%
Student > Master 4 11%
Other 3 9%
Student > Bachelor 2 6%
Other 6 17%
Unknown 3 9%
Readers by discipline Count As %
Chemistry 14 40%
Agricultural and Biological Sciences 6 17%
Biochemistry, Genetics and Molecular Biology 4 11%
Engineering 3 9%
Nursing and Health Professions 1 3%
Other 4 11%
Unknown 3 9%