Chapter title |
Derivatization in Capillary Electrophoresis.
|
---|---|
Chapter number | 3 |
Book title |
Capillary Electrophoresis
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-6403-1_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6401-7, 978-1-4939-6403-1
|
Authors |
M. Luisa Marina, María Castro-Puyana |
Editors |
Philippe Schmitt-Kopplin |
Abstract |
Capillary electrophoresis is a well-established separation technique in analytical research laboratories worldwide. Its interesting advantages make CE an efficient and potent alternative to other chromatographic techniques. However, it is also recognized that its main drawback is the relatively poor sensitivity when using optical detection. One way to overcome this limitation is to perform a derivatization reaction which is intended to provide the analyte more suitable analytical characteristics enabling a high sensitive detection. Based on the analytical step where the CE derivatization takes place, it can be classified as precapillary (before separation), in-capillary (during separation), or postcapillary (after separation). This chapter describes the application of four different derivatization protocols (in-capillary and precapillary modes) to carry out the achiral and chiral analysis of different compounds in food and biological samples with three different detection modes (UV, LIF, and MS). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 3 | 9% |
Unknown | 32 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 29% |
Researcher | 5 | 14% |
Student > Master | 4 | 11% |
Student > Bachelor | 3 | 9% |
Other | 3 | 9% |
Other | 6 | 17% |
Unknown | 4 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 14 | 40% |
Agricultural and Biological Sciences | 5 | 14% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Engineering | 3 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 4 | 11% |
Unknown | 4 | 11% |