| Chapter title |
A Semiempirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free Zone Electrophoresis.
|
|---|---|
| Chapter number | 2 |
| Book title |
Capillary Electrophoresis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6403-1_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6401-7, 978-1-4939-6403-1
|
| Authors |
Philippe Schmitt-Kopplin, Agnes Fekete |
| Editors |
Philippe Schmitt-Kopplin |
| Abstract |
A phenomenological model is proposed for the evaluation of relative electrophoretic migration of charged substances present in mixtures and for the rapid pH optimization prior CZE method development. The simple and robust model is based on the Offord model that takes account of the chemical structure. The effective charge and the molecular mass of the molecule are needed; the charge can easily be calculated from pK a obtained from known sources or simulated with existing pK-calculation programs. A first example was chosen with the separation of hydroxy-s-triazines to illustrate the applicability of this simple approach for determination of the first buffer-pH conditions prior experimental method optimization when separation of different ions is needed. In a second example, the confirmation of aminoalcohols in the CZE method development of unsaturated hexahydro-triazines and oxasolidines. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Student > Master | 1 | 17% |
| Researcher | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Chemistry | 1 | 17% |
| Unknown | 3 | 50% |