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Mendeley readers
| Chapter title |
Simultaneous RNA-DNA FISH.
|
|---|---|
| Chapter number | 11 |
| Book title |
Long Non-Coding RNAs
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3378-5_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3376-1, 978-1-4939-3378-5
|
| Authors |
Lan-Tian Lai, Zhenyu Meng, Fangwei Shao, Li-Feng Zhang |
| Editors |
Yi Feng, Lin Zhang |
| Abstract |
A highly useful tool for studying lncRNAs is simultaneous RNA-DNA FISH, which reveals the localization and quantitative information of RNA and DNA in cellular contexts. However, a simple combination of RNA FISH and DNA FISH often generates disappointing results because the fragile RNA signals are often damaged by the harsh conditions used in DNA FISH for denaturing the DNA. Here, we describe a robust and simple RNA-DNA FISH protocol, in which amino-labeled nucleic acid probes are used for RNA FISH. The method is suitable to detect single-RNA molecules simultaneously with DNA. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Singapore | 1 | 8% |
| Unknown | 11 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 25% |
| Student > Bachelor | 2 | 17% |
| Researcher | 2 | 17% |
| Professor | 1 | 8% |
| Other | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 33% |
| Immunology and Microbiology | 2 | 17% |
| Neuroscience | 2 | 17% |
| Agricultural and Biological Sciences | 1 | 8% |
| Chemical Engineering | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 1 | 8% |