| Chapter title |
Emerging Methods in Chemoproteomics with Relevance to Drug Discovery.
|
|---|---|
| Chapter number | 2 |
| Book title |
Cancer Gene Networks
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6539-7_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6537-3, 978-1-4939-6539-7
|
| Authors |
Chuong Nguyen, Graham M. West, Kieran F. Geoghegan |
| Editors |
Usha Kasid, Robert Clarke |
| Abstract |
A powerful interplay exists between the recognition of gene families, sensitive techniques in proteomics, and the interrogation of protein function using chemical probes. The most prominent methods, such as affinity capture, activity-based protein profiling and photoaffinity labeling, are extensively reviewed in the literature. Here we briefly review additional methods developed in the past 15 years. These include "stability proteomics" methods such as proteomically analyzed cellular thermal shift assays and the use of chemical oxidation as a probe of structure, the use of multiple bead-linked kinase inhibitors to analyze inhibitor specificities, and advances in the use of proteolysis-targeting chimeras for selective protein elimination. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 24% |
| Student > Ph. D. Student | 7 | 18% |
| Other | 3 | 8% |
| Lecturer | 1 | 3% |
| Student > Doctoral Student | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 14 | 37% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 16% |
| Chemistry | 6 | 16% |
| Agricultural and Biological Sciences | 3 | 8% |
| Neuroscience | 3 | 8% |
| Medicine and Dentistry | 3 | 8% |
| Other | 4 | 11% |
| Unknown | 13 | 34% |