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Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

Overview of attention for book
Cover of 'Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation'

Table of Contents

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    Book Overview
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    Chapter 1 Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators.
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    Chapter 2 Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins.
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    Chapter 3 Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos.
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    Chapter 4 Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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    Chapter 5 Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos.
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    Chapter 6 Establishment of Time- and Cell-Specific RNAi in Caenorhabditis elegans.
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    Chapter 7 Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing.
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    Chapter 8 Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets.
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    Chapter 9 eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response.
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    Chapter 10 High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine.
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    Chapter 11 Accurate Detection of Differential Expression and Splicing Using Low-Level Features.
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    Chapter 12 Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.
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    Chapter 13 Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae.
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    Chapter 14 Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract.
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    Chapter 15 Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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    Chapter 16 Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells.
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    Chapter 17 In Vitro Assay to Study Histone Ubiquitination During Transcriptional Regulation.
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    Chapter 18 Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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    Chapter 19 Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor Gene.
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    Chapter 20 Transcriptional Analysis-Based Integrative Genomics Approach to Identify Tumor-Promoting Metabolic Genes.
Attention for Chapter 10: High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine.
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  • High Attention Score compared to outputs of the same age and source (82nd percentile)

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Chapter title
High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine.
Chapter number 10
Book title
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6518-2_10
Pubmed ID
Book ISBNs
978-1-4939-6516-8, 978-1-4939-6518-2
Authors

Andrzej J. Rutkowski, Lars Dölken

Editors

Narendra Wajapeyee, Romi Gupta

Abstract

Cellular RNA levels are orchestrated by highly regulated processes involving RNA synthesis (transcription), processing (e.g., splicing, polyadenylation, transport), and degradation. Profiling these changes provides valuable information on the regulation of gene expression. Total cellular RNA is a poor template for revealing short-term changes in gene expression, alterations in RNA decay rates, and the kinetics of RNA processing as well as the differentiation thereof. Here, we describe the metabolic labeling and purification of newly transcribed RNA with 4-thiouridine, by which these limitations are overcome.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 35%
Professor 3 15%
Student > Bachelor 2 10%
Researcher 2 10%
Student > Master 2 10%
Other 1 5%
Unknown 3 15%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 40%
Agricultural and Biological Sciences 4 20%
Engineering 2 10%
Environmental Science 1 5%
Medicine and Dentistry 1 5%
Other 1 5%
Unknown 3 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2016.
All research outputs
#5,765,859
of 22,901,818 outputs
Outputs from Methods in molecular biology
#1,616
of 13,134 outputs
Outputs of similar age
#106,738
of 420,455 outputs
Outputs of similar age from Methods in molecular biology
#183
of 1,074 outputs
Altmetric has tracked 22,901,818 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 13,134 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,455 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.