Chapter title |
Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.
|
---|---|
Chapter number | 12 |
Book title |
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6518-2_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6516-8, 978-1-4939-6518-2
|
Authors |
Mowei Zhou, Si Wu, David L. Stenoien, Zhaorui Zhang, Lanelle Connolly, Michael Freitag, Ljiljana Paša-Tolić |
Editors |
Narendra Wajapeyee, Romi Gupta |
Abstract |
Top-down mass spectrometry is a valuable tool for understanding gene expression through characterization of combinatorial histone post-translational modifications (i.e., histone code). In this protocol, we describe a top-down workflow that employs liquid chromatography (LC) coupled to mass spectrometry (MS), for fast global profiling of changes in histone proteoforms, and apply LCMS top-down approach for comparative analysis of a wild-type and a mutant fungal species. The proteoforms exhibiting differential abundances can be subjected to further targeted studies by other MS or orthogonal (e.g., biochemical) assays. This method can be generally adapted for screening of changes in histone modifications between samples such as wild type vs. mutant or healthy vs. diseased. |
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