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Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation

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Cover of 'Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation'

Table of Contents

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    Book Overview
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    Chapter 1 Fluorescence Reporter-Based Genome-Wide RNA Interference Screening to Identify Alternative Splicing Regulators.
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    Chapter 2 Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins.
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    Chapter 3 Efficient Preparation of High-Complexity ChIP-Seq Profiles from Early Xenopus Embryos.
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    Chapter 4 Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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    Chapter 5 Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos.
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    Chapter 6 Establishment of Time- and Cell-Specific RNAi in Caenorhabditis elegans.
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    Chapter 7 Cell-Penetrating Peptide-Mediated Delivery of Cas9 Protein and Guide RNA for Genome Editing.
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    Chapter 8 Epigenetic Analysis of Endocrine Cell Subtypes from Human Pancreatic Islets.
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    Chapter 9 eIF3 Regulation of Protein Synthesis, Tumorigenesis, and Therapeutic Response.
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    Chapter 10 High-Resolution Gene Expression Profiling of RNA Synthesis, Processing, and Decay by Metabolic Labeling of Newly Transcribed RNA Using 4-Thiouridine.
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    Chapter 11 Accurate Detection of Differential Expression and Splicing Using Low-Level Features.
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    Chapter 12 Profiling Changes in Histone Post-translational Modifications by Top-Down Mass Spectrometry.
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    Chapter 13 Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae.
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    Chapter 14 Optimizing In Vitro Pre-mRNA 3' Cleavage Efficiency: Reconstitution from Anion-Exchange Separated HeLa Cleavage Factors and from Adherent HeLa Cell Nuclear Extract.
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    Chapter 15 Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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    Chapter 16 Using an Inducible CRISPR-dCas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells.
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    Chapter 17 In Vitro Assay to Study Histone Ubiquitination During Transcriptional Regulation.
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    Chapter 18 Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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    Chapter 19 Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor Gene.
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    Chapter 20 Transcriptional Analysis-Based Integrative Genomics Approach to Identify Tumor-Promoting Metabolic Genes.
Attention for Chapter 2: Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins.
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Chapter title
Tandem Affinity Purification Approach Coupled to Mass Spectrometry to Identify Post-translational Modifications of Histones Associated with Chromatin-Binding Proteins.
Chapter number 2
Book title
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6518-2_2
Pubmed ID
Book ISBNs
978-1-4939-6516-8, 978-1-4939-6518-2
Authors

Sophie Beyer, Philippe Robin, Slimane Ait-Si-Ali

Editors

Narendra Wajapeyee, Romi Gupta

Abstract

Protein purification by tandem affinity purification (TAP)-tag coupled to mass spectrometry analysis is usually used to reveal protein complex composition. Here we describe a TAP-tag purification of chromatin-bound proteins along with associated nucleosomes, which allow exhaustive identification of protein partners. Moreover, this method allows exhaustive identification of the post-translational modifications (PTMs) of the associated histones. Thus, in addition to partner characterization, this approach reveals the associated epigenetic landscape that can shed light on the function and properties of the studied chromatin-bound protein.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Student > Bachelor 1 33%
Student > Postgraduate 1 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 67%
Biochemistry, Genetics and Molecular Biology 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2018.
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#20,353,668
of 22,901,818 outputs
Outputs from Methods in molecular biology
#9,922
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Outputs of similar age
#355,334
of 420,455 outputs
Outputs of similar age from Methods in molecular biology
#845
of 1,074 outputs
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