| Chapter title |
Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor Gene.
|
|---|---|
| Chapter number | 19 |
| Book title |
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6518-2_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6516-8, 978-1-4939-6518-2
|
| Authors |
Matteo Forloni, Thuy Ho, Lisha Sun, Narendra Wajapeyee, Forloni, Matteo, Ho, Thuy, Sun, Lisha, Wajapeyee, Narendra |
| Editors |
Narendra Wajapeyee, Romi Gupta |
| Abstract |
RNA interference (RNAi) is a powerful research tool that can be used to silence the expression of a specific gene. In the past several years, RNAi has provided the opportunity to identify factors and pathways involved in complex biological processes by performing unbiased loss-of-function screens on a genome-wide scale. Here we describe a genome-wide RNAi screening strategy to identify factors that regulates epigenetic silencing of a specific tumor suppressor gene, using RASSF1A as an example. The approach we describe is a general RNAi screening strategy that can be applied to identify other factors that drive and/or maintain epigenetic modifications on specific genes, including cancer-related genes. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 40% |
| Professor | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Student > Master | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 100% |