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Mendeley readers
Chapter title |
Determining if an mRNA is a Substrate of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae.
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Chapter number | 13 |
Book title |
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6518-2_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6516-8, 978-1-4939-6518-2
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Authors |
Marcus J. O. Johansson |
Editors |
Narendra Wajapeyee, Romi Gupta |
Abstract |
Nonsense-mediated mRNA decay (NMD) is a conserved eukaryotic quality control mechanism which triggers decay of mRNAs harboring premature translation termination codons. In this chapter, I describe methods for monitoring the influence of NMD on mRNA abundance and decay rates in Saccharomyces cerevisiae. The descriptions include detailed methods for growing yeast cells, total RNA isolation, and Northern blotting. Although the chapter focuses on NMD, the methods can be easily adapted to assess the effect of other mRNA decay pathways. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 50% |
Student > Ph. D. Student | 1 | 25% |
Unknown | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Unknown | 1 | 25% |