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Chapter title |
Assay for Transposase-Accessible Chromatin with High-Throughput Sequencing (ATAC-Seq) Protocol for Zebrafish Embryos.
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Chapter number | 5 |
Book title |
Eukaryotic Transcriptional and Post-Transcriptional Gene Expression Regulation
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6518-2_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6516-8, 978-1-4939-6518-2
|
Authors |
Canan Doganli, Melissa Sandoval, Sean Thomas, Daniel Hart |
Editors |
Narendra Wajapeyee, Romi Gupta |
Abstract |
Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) is a useful method to map genome-wide chromatin accessibility and nucleosome positioning. Genome-wide sequencing is performed utilizing adapter sequences inserted by a prokaryotic transposase, Tn5, into the accessible regions of chromatin. Here we describe the use of ATAC-seq in the zebrafish embryo and thereby the applicability of this approach in whole vertebrate embryos. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 23% |
Student > Master | 4 | 13% |
Student > Bachelor | 3 | 10% |
Professor > Associate Professor | 3 | 10% |
Researcher | 3 | 10% |
Other | 8 | 27% |
Unknown | 2 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 12 | 40% |
Agricultural and Biological Sciences | 6 | 20% |
Medicine and Dentistry | 3 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 7% |
Social Sciences | 1 | 3% |
Other | 3 | 10% |
Unknown | 3 | 10% |