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Exosomes and Microvesicles

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Cover of 'Exosomes and Microvesicles'

Table of Contents

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    Book Overview
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    Chapter 1 Methods to Analyze EVs.
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    Chapter 2 Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
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    Chapter 3 Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
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    Chapter 4 Imaging and Quantification of Extracellular Vesicles by Transmission Electron Microscopy.
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    Chapter 5 Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.
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    Chapter 6 Small RNA Library Construction for Exosomal RNA from Biological Samples for the Ion Torrent PGM™ and Ion S5™ System.
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    Chapter 7 A Protocol for Isolation and Proteomic Characterization of Distinct Extracellular Vesicle Subtypes by Sequential Centrifugal Ultrafiltration.
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    Chapter 8 Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array).
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    Chapter 9 Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation.
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    Chapter 10 A Method for Isolation of Extracellular Vesicles and Characterization of Exosomes from Brain Extracellular Space.
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    Chapter 11 Isolation of Exosomes and Microvesicles from Cell Culture Systems to Study Prion Transmission.
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    Chapter 12 Isolation of Platelet-Derived Extracellular Vesicles.
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    Chapter 13 Bioinformatics Tools for Extracellular Vesicles Research.
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    Chapter 14 Preparation and Isolation of siRNA-Loaded Extracellular Vesicles.
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    Chapter 15 Interaction of Extracellular Vesicles with Endothelial Cells Under Physiological Flow Conditions.
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    Chapter 16 Flow Cytometric Analysis of Extracellular Vesicles.
Attention for Chapter 9: Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation.
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Chapter title
Purification and Analysis of Exosomes Released by Mature Cortical Neurons Following Synaptic Activation.
Chapter number 9
Book title
Exosomes and Microvesicles
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6728-5_9
Pubmed ID
Book ISBNs
978-1-4939-6726-1, 978-1-4939-6728-5
Authors

Karine Laulagnier, Charlotte Javalet, Fiona J. Hemming, Rémy Sadoul

Editors

Andrew F Hill

Abstract

Exosomes are vesicles released by most cells into their environment upon fusion of multivesicular endosomes with the plasma membrane. Exosomes are vesicles of 60-100 nm in diameter, floating in sucrose at a density of ~1.15 g/mL and carrying a number of marker proteins such as Alix, Tsg101, and Flotillin-1. We use dissociated cortical neurons cultured for around two weeks as exosome-releasing cells. In these conditions, neurons make mature synapses and form networks that can be activated by physiological stimuli. Here, we describe methods to culture differentiated cortical neurons, induce exosome release by increasing glutamatergic synapse activity, and purify exosomes by differential centrifugations followed by density separation using sucrose gradients. These protocols allow purification of neuronal exosomes released within minutes of activation of glutamatergic synapses.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 45%
Researcher 2 18%
Student > Doctoral Student 2 18%
Student > Master 1 9%
Unknown 1 9%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 36%
Agricultural and Biological Sciences 2 18%
Chemical Engineering 1 9%
Medicine and Dentistry 1 9%
Neuroscience 1 9%
Other 0 0%
Unknown 2 18%