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Food Allergens

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Cover of 'Food Allergens'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of the Commonly Used Methods for Food Allergens
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    Chapter 2 Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques
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    Chapter 3 Recombinant Allergen Production in E. coli
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    Chapter 4 Recombinant Allergens Production in Yeast
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    Chapter 5 2D-Electrophoresis and Immunoblotting in Food Allergy
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    Chapter 6 Two-Dimensional Electrophoresis and Identification by Mass Spectrometry
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    Chapter 7 Enzyme-Linked Immunosorbent Assay (ELISA)
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    Chapter 8 Detection of Food Allergens by Taqman Real-Time PCR Methodology
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    Chapter 9 Detection of Food Allergens by Phage-Displayed Produced Antibodies
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    Chapter 10 Protein Microarray-Based IgE Immunoassay for Allergy Diagnosis
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    Chapter 11 Basophil Degranulation Assay
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    Chapter 12 Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy
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    Chapter 13 Assessment of IgE Reactivity of β-Casein by Western Blotting After Digestion with Simulated Gastric Fluid
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    Chapter 14 IgE Epitope Mapping Using Peptide Microarray Immunoassay
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    Chapter 15 T-Cell Proliferation Assay: Determination of Immunodominant T-Cell Epitopes of Food Allergens
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    Chapter 16 Tetramer-Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens
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    Chapter 17 T-Cell Epitope Prediction
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    Chapter 18 An Overview of Bioinformatics Tools and Resources in Allergy
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    Chapter 19 The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid-Loaded Dendritic Cells
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    Chapter 20 Recent Advances in the Detection of Allergens in Foods
Attention for Chapter 5: 2D-Electrophoresis and Immunoblotting in Food Allergy
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Chapter title
2D-Electrophoresis and Immunoblotting in Food Allergy
Chapter number 5
Book title
Food Allergens
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6925-8_5
Pubmed ID
Book ISBNs
978-1-4939-6923-4, 978-1-4939-6925-8
Authors

Galina Grishina, Luda Bardina, Alexander Grishin

Editors

Jing Lin, Marcos Alcocer

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2DE) and Western immunoblotting have proven to be invaluable and fundamental techniques for comprehensive characterization of food allergens. Here, we describe and discuss detailed protocols used in our studies on identification of allergenic proteins in shrimp, sesame, hazelnut, and pistachio.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 19%
Student > Ph. D. Student 3 14%
Student > Bachelor 3 14%
Student > Postgraduate 1 5%
Unknown 10 48%
Readers by discipline Count As %
Medicine and Dentistry 3 14%
Agricultural and Biological Sciences 3 14%
Biochemistry, Genetics and Molecular Biology 2 10%
Chemistry 2 10%
Energy 1 5%
Other 1 5%
Unknown 9 43%