| Chapter title |
Enzyme-Linked Immunosorbent Assay (ELISA)
|
|---|---|
| Chapter number | 7 |
| Book title |
Food Allergens
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6925-8_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6923-4, 978-1-4939-6925-8
|
| Authors |
George N. Konstantinou, Konstantinou, George N. |
| Editors |
Jing Lin, Marcos Alcocer |
| Abstract |
Food allergy is a public health concern especially after recognizing its constantly increased prevalence and severity. Despite careful reading of food ingredient statements, food allergic individuals may experience reactions caused by "hidden", "masked", or "contaminated" proteins that are known major allergens. Many techniques have been developed to detect even small traces of food allergens, for clinical or laboratory purposes. Enzyme-linked immunosorbent assay (ELISA) is one of the best validated and most routinely used immunoassay in allergy research, in allergy diagnosis in allergy-related quality control in various industries. Although as a technique it has been implemented for the last 45 years, the evolution in biochemistry allowed the development of ultrasensitive ELISA variations that are capable of measuring quantities in the scale of picograms, rendering ELISA attractive, robust, and very famous. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Japan | 1 | <1% |
| Unknown | 422 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 97 | 23% |
| Student > Master | 52 | 12% |
| Student > Ph. D. Student | 34 | 8% |
| Researcher | 21 | 5% |
| Student > Doctoral Student | 12 | 3% |
| Other | 38 | 9% |
| Unknown | 170 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 74 | 17% |
| Agricultural and Biological Sciences | 34 | 8% |
| Medicine and Dentistry | 32 | 8% |
| Chemistry | 20 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 18 | 4% |
| Other | 60 | 14% |
| Unknown | 186 | 44% |