| Chapter title |
Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
|
|---|---|
| Chapter number | 7 |
| Book title |
Heterologous Protein Production in CHO Cells
|
| Published in |
Methods in molecular biology, May 2017
|
| DOI | 10.1007/978-1-4939-6972-2_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6971-5, 978-1-4939-6972-2
|
| Authors |
Grav, Lise Marie, la Cour Karottki, Karen Julie, Lee, Jae Seong, Kildegaard, Helene Faustrup, Lise Marie Grav, Karen Julie la Cour Karottki, Jae Seong Lee, Helene Faustrup Kildegaard |
| Editors |
Paula Meleady |
| Abstract |
Genome editing has become an increasingly important aspect of Chinese Hamster Ovary (CHO ) cell line engineering for improving production of recombinant protein therapeutics. Currently, the focus is directed toward expanding the product diversity, controlling and improving product quality and yields. In this chapter, we present our protocol on how to use the genome editing tool Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) to knockout engineering target genes in CHO cells. As an example, we refer to the glutamine synthetase (GS)-encoding gene as the knockout target gene, a knockout that increases the selection efficiency of the GS-mediated gene amplification system. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 11 | 21% |
| Student > Master | 10 | 19% |
| Researcher | 7 | 13% |
| Student > Ph. D. Student | 5 | 10% |
| Student > Doctoral Student | 1 | 2% |
| Other | 5 | 10% |
| Unknown | 13 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 38% |
| Agricultural and Biological Sciences | 4 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 8% |
| Chemical Engineering | 2 | 4% |
| Engineering | 2 | 4% |
| Other | 6 | 12% |
| Unknown | 14 | 27% |