| Chapter title |
Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture
|
|---|---|
| Chapter number | 14 |
| Book title |
Heterologous Protein Production in CHO Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6972-2_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6971-5, 978-1-4939-6972-2
|
| Authors |
Yuzhou Fan, Helene Faustrup Kildegaard, Mikael Rørdam Andersen |
| Editors |
Paula Meleady |
| Abstract |
Chinese hamster ovary (CHO) cells have become the primary expression system for the production of complex recombinant proteins due to their long-term success in industrial scale production and generating appropriate protein N-glycans similar to that of humans. Control and optimization of protein N-glycosylation is crucial, as the structure of N-glycans can largely influence both biological and physicochemical properties of recombinant proteins. Protein N-glycosylation in CHO cell culture can be controlled and tuned by engineering medium, feed, culture process, as well as genetic elements of the cell. In this chapter, we will focus on how to carry out experiments for N-glycosylation modulation through medium and feed optimization. The workflow and typical methods involved in the experiment process will be presented. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 1 | 5% |
| Unknown | 21 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 23% |
| Student > Doctoral Student | 3 | 14% |
| Student > Ph. D. Student | 2 | 9% |
| Student > Bachelor | 2 | 9% |
| Professor > Associate Professor | 2 | 9% |
| Other | 1 | 5% |
| Unknown | 7 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 23% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 14% |
| Agricultural and Biological Sciences | 3 | 14% |
| Chemical Engineering | 2 | 9% |
| Computer Science | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 8 | 36% |