Report for: Heterologous Protein Production in CHO Cells

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Chapter title	Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells
Chapter number	12
Book title	Heterologous Protein Production in CHO Cells
Published in	Methods in molecular biology, January 2017
DOI	10.1007/978-1-4939-6972-2_12
Pubmed ID	28493131
Book ISBNs	978-1-4939-6971-5, 978-1-4939-6972-2
Authors	Orla Coleman, Michael Henry, Martin Clynes, Paula Meleady, Coleman, Orla, Henry, Michael, Clynes, Martin, Meleady, Paula
Editors	Paula Meleady
Abstract	Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell line for biopharmaceutical production because of their ability to correctly fold and posttranslationally modify recombinant proteins that are compatible with human use. Proteomics, along with other 'omic platforms, are being used to understand the biology of CHO cells with the ultimate aim of enhancing CHO cell factories for more efficient production of biopharmaceuticals. In this chapter, we will describe an efficient protocol called Filter Aided Sample Preparation (FASP) for the extraction of proteins from CHO cells for proteomic studies. FASP uses a common ultrafiltration device whereby the membrane pores are small enough to allow contaminating detergents to pass through, while proteins are too large and are retained and concentrated in the filter unit. This method of sample preparation and protein digestion is universally applicable and can be easily employed in any proteomics facilities as standard everyday laboratory reagents and equipment are used.

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Geographical breakdown

Country	Count	As %
Unknown	1	100%

Demographic breakdown

Type	Count	As %
Members of the public	1	100%

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country	Count	As %
Unknown	19	100%

Demographic breakdown

Readers by professional status	Count	As %
Researcher	3	16%
Other	2	11%
Student > Postgraduate	2	11%
Student > Ph. D. Student	2	11%
Student > Bachelor	1	5%
Other	2	11%
Unknown	7	37%

Readers by discipline	Count	As %
Biochemistry, Genetics and Molecular Biology	7	37%
Agricultural and Biological Sciences	2	11%
Unspecified	1	5%
Unknown	9	47%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 February 2018.

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#18,547,867

of 22,971,207 outputs

Outputs from Methods in molecular biology

#7,945

of 13,146 outputs

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#311,290

of 421,094 outputs

Outputs of similar age from Methods in molecular biology

#693

of 1,074 outputs

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Heterologous Protein Production in CHO Cells

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