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Heterologous Protein Production in CHO Cells

Overview of attention for book
Cover of 'Heterologous Protein Production in CHO Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Cells
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    Chapter 2 Glycoengineering of CHO Cells to Improve Product Quality
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    Chapter 3 Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension
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    Chapter 4 Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression
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    Chapter 5 Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells
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    Chapter 6 Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors
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    Chapter 7 Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
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    Chapter 8 Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture
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    Chapter 9 Selection of High-Producing Clones Using FACS for CHO Cell Line Development
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    Chapter 10 The ‘Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity
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    Chapter 11 A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data
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    Chapter 12 Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells
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    Chapter 13 Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells
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    Chapter 14 Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture
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    Chapter 15 Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells
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    Chapter 16 Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses
Attention for Chapter 6: Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors
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Chapter title
Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors
Chapter number 6
Book title
Heterologous Protein Production in CHO Cells
Published in
Methods in molecular biology, May 2017
DOI 10.1007/978-1-4939-6972-2_6
Pubmed ID
28493125
Book ISBNs
978-1-4939-6971-5, 978-1-4939-6972-2
Authors

Costello, Alan, Lao, Nga, Clynes, Martin, Barron, Niall, Alan Costello, Nga Lao, Martin Clynes, Niall Barron

Editors

Paula Meleady

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs of about 22 nucleotides in length and have proven to be useful targets for genetic modifications for desirable phenotype in the biotech industry. The use of constitutively expressed "miRNA sponge" vectors in which multiple, tandem miRNA binding sites containing transcripts are transcriptionally regulated by a constitutive promoter for down regulating the levels of endogenous microRNAs in Chinese hamster ovary (CHO) cells has shown to be more advantageous than using synthetic antisense oligonucleotides. The application of miRNA sponges in biotechnological processes, however, could be more effective, if expression of miRNA sponges could be tuned. In this chapter, we present a method for the generation of stable CHO cell lines expressing a TET-ON-SanDI-miRNA-sponge that is in theory expressed only in the presence of an inducer.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 29%
Student > Ph. D. Student 2 14%
Student > Bachelor 1 7%
Student > Doctoral Student 1 7%
Professor 1 7%
Other 0 0%
Unknown 5 36%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 57%
Agricultural and Biological Sciences 1 7%
Unknown 5 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2017.
All research outputs
#17,892,691
of 22,971,207 outputs
Outputs from Methods in molecular biology
#7,269
of 13,146 outputs
Outputs of similar age
#222,293
of 310,860 outputs
Outputs of similar age from Methods in molecular biology
#176
of 305 outputs
Altmetric has tracked 22,971,207 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,146 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 310,860 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 305 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.

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