| Chapter title |
Strategies and Considerations for Improving Expression of “Difficult to Express” Proteins in CHO Cells
|
|---|---|
| Chapter number | 1 |
| Book title |
Heterologous Protein Production in CHO Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6972-2_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6971-5, 978-1-4939-6972-2
|
| Authors |
Christina S. Alves, Terrence M. Dobrowsky |
| Editors |
Paula Meleady |
| Abstract |
Despite substantial advances in the field of mammalian expression, there are still proteins that are characterized as difficult to express. Determining the expression bottleneck requires troubleshooting techniques specific for the given molecule and host. The complex array of intracellular processes involved in protein expression includes transcription, protein folding, post-translation processing, and secretion. Challenges in any of these steps could result in low protein expression, while the inherent properties of the molecule itself may limit its production via mechanisms such as cytotoxicity or inherent instability. Strategies to identify the rate-limiting step and subsequently improve expression and production are discussed here. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 24% |
| Student > Ph. D. Student | 8 | 20% |
| Student > Master | 6 | 15% |
| Researcher | 5 | 12% |
| Student > Doctoral Student | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 9 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 23 | 56% |
| Agricultural and Biological Sciences | 4 | 10% |
| Engineering | 3 | 7% |
| Chemistry | 1 | 2% |
| Business, Management and Accounting | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 9 | 22% |