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Mendeley readers
| Chapter title |
Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells
|
|---|---|
| Chapter number | 5 |
| Book title |
Heterologous Protein Production in CHO Cells
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6972-2_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6971-5, 978-1-4939-6972-2
|
| Authors |
Eric Baek, Soo Min Noh, Gyun Min Lee |
| Editors |
Paula Meleady |
| Abstract |
Improving the time integral of viable cell concentration by overcoming cell death, namely apoptosis, is one of the widely used strategies for efficient production of therapeutic proteins. By establishing stable cell lines that overexpress anti-apoptotic genes or down-regulate pro-apoptotic genes, the final product yields can be enhanced as cells become more resistance to environmental stresses. From the selection of high-expressing clones to verification of anti-apoptotic activity, the method to construct a stable anti-apoptotic cell line is discussed in this chapter. |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 15% |
| Student > Master | 4 | 15% |
| Researcher | 3 | 11% |
| Student > Ph. D. Student | 3 | 11% |
| Student > Doctoral Student | 2 | 7% |
| Other | 2 | 7% |
| Unknown | 9 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 33% |
| Agricultural and Biological Sciences | 3 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 7% |
| Engineering | 2 | 7% |
| Unknown | 11 | 41% |