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Chapter title |
Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension
|
---|---|
Chapter number | 3 |
Book title |
Heterologous Protein Production in CHO Cells
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6972-2_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6971-5, 978-1-4939-6972-2
|
Authors |
Yashas Rajendra, Sowmya Balasubramanian, David L. Hacker |
Editors |
Paula Meleady |
Abstract |
We describe a one-liter transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells using polyethyleneimine (PEI) for DNA delivery. The method involves transfection at a high cell density (5 × 10(6) cells/mL) by direct addition of plasmid DNA (pDNA) and PEI to the culture and subsequent incubation at 31 °C with agitation by orbital shaking. We also describe an alternative method in which 90% of the pDNA is replaced by nonspecific (filler) DNA, and the production phase is performed at 31 °C in the presence of 0.25% N, N-dimethylacetamide (DMA). |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 15 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 3 | 20% |
Student > Bachelor | 3 | 20% |
Researcher | 3 | 20% |
Student > Master | 2 | 13% |
Student > Doctoral Student | 1 | 7% |
Other | 0 | 0% |
Unknown | 3 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 53% |
Agricultural and Biological Sciences | 2 | 13% |
Materials Science | 1 | 7% |
Chemistry | 1 | 7% |
Unknown | 3 | 20% |