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Mendeley readers
Chapter title |
Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre
|
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Chapter number | 7 |
Book title |
Site-Specific Recombinases
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-7169-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
Authors |
Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, Michael T. Chin |
Abstract |
The technique of Cre-mediated DNA recombination at loxP sites has been used widely in manipulation of the genome in cultured cells and in living organisms. Local delivery of Cre recombinase protein tagged with a cell-penetrating (or permeable) peptide (Cre-CPP) has the advantage of additional spatial and temporal control when compared to genetic delivery methods. In this chapter, we describe protocols for injection-based intramuscular delivery of Cre-CPP dissolved in hydrogel to skeletal muscle and by ultrasound-guided injection to cardiac muscle in mice. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Postgraduate | 1 | 25% |
Unknown | 3 | 75% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Unknown | 3 | 75% |