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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways
|
|---|---|
| Chapter number | 18 |
| Book title |
Site-Specific Recombinases
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7169-5_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
| Authors |
Wei Liu, Laura R. Tuck, Jon Marles Wright, Yizhi Cai, Laura R Tuck |
| Abstract |
The site-specific recombinase Cre was previously reported to have in vitro activity. Here, we describe the method of purifying two new tyrosine site-specific recombinases VCre and Dre along with Cre by nickel affinity chromatography. We proved the in vitro function of the VCre and Dre on their respective conditional recombination sites. We also developed a methodology to one-step construct and optimize the productivity of a biosynthetic pathway through the combinatorial integration of promoters into a plasmid-encoded pathway by simply incubating a DNA mixture with recombinase system at 37 °C in vitro. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 5 | 83% |
| Unknown | 1 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 5 | 83% |
| Members of the public | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 23% |
| Student > Ph. D. Student | 3 | 23% |
| Other | 2 | 15% |
| Lecturer | 1 | 8% |
| Lecturer > Senior Lecturer | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 38% |
| Agricultural and Biological Sciences | 3 | 23% |
| Computer Science | 1 | 8% |
| Engineering | 1 | 8% |
| Unknown | 3 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2024.
All research outputs
#8,289,834
of 25,641,627 outputs
Outputs from Methods in molecular biology
#2,540
of 14,313 outputs
Outputs of similar age
#140,188
of 423,289 outputs
Outputs of similar age from Methods in molecular biology
#247
of 1,088 outputs
Altmetric has tracked 25,641,627 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 14,313 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 423,289 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 1,088 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.