Chapter title |
Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay
|
---|---|
Chapter number | 4 |
Book title |
Chaperones
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7477-1_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7476-4, 978-1-4939-7477-1
|
Authors |
Mikko Taipale |
Abstract |
Chaperones associate with hundreds or thousands of diverse client proteins and regulate their function. Chaperone/client interactions are generally very transient and involve a highly orchestrated assembly and disassembly of regulatory co-factors. This poses specific challenges for identifying and characterizing these interactions in a scalable and sensitive manner. LUMIER assay, which takes advantage of the high sensitivity and linear range of luminescence-based detection, has proven to be an ideal assay to quantitatively profile chaperone/client interactions in a high-throughput manner. This article provides step-by-step instructions for quantitatively profiling these interactions with LUMIER. |
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Unspecified | 1 | 7% |
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