↓ Skip to main content
Embed badge What is this page?

Chaperones

Overview of attention for book
Cover of 'Chaperones'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Targeted Deletion of Hsf1, 2, and 4 Genes in Mice
  3. Altmetric Badge
    Chapter 2 Role of Heat Shock Factors in Stress-Induced Transcription
  4. Altmetric Badge
    Chapter 3 Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
  5. Altmetric Badge
    Chapter 4 Quantitative Profiling of Chaperone/Client Interactions with LUMIER Assay
  6. Altmetric Badge
    Chapter 5 Measurement of Chaperone-Mediated Effects on Polyglutamine Protein Aggregation by the Filter Trap Assay
  7. Altmetric Badge
    Chapter 6 Fluorescent-Linked Enzyme Chemoproteomic Strategy (FLECS) for Identifying HSP70 Inhibitors
  8. Altmetric Badge
    Chapter 7 A High-Throughput Screen for Inhibitors of the Hsp90-Chaperone Machine
  9. Altmetric Badge
    Chapter 8 Primary Colorectal Cells Culture as a Translational Research Model
  10. Altmetric Badge
    Chapter 9 Cell Death and Survival Assays
  11. Altmetric Badge
    Chapter 10 Detecting the Potential Pharmacological Synergy of Drug Combination by Viability Assays In Vitro
  12. Altmetric Badge
    Chapter 11 Proteomic Profiling of Hsp90 Inhibitors
  13. Altmetric Badge
    Chapter 12 Analysis of HspB1 (Hsp27) Oligomerization and Phosphorylation Patterns and Its Interaction with Specific Client Polypeptides
  14. Altmetric Badge
    Chapter 13 Nucleotide Exchange Factors for Hsp70 Chaperones
  15. Altmetric Badge
    Chapter 14 Determination of Hsp90 Activity Through Activation of Glucocorticoid Receptors in Yeast
  16. Altmetric Badge
    Chapter 15 Bacterial Hsp90 ATPase Assays
  17. Altmetric Badge
    Chapter 16 Detecting Posttranslational Modifications of Hsp90
  18. Altmetric Badge
    Chapter 17 Chromatin Immunoprecipitation (ChIP) of Heat Shock Protein 90 (Hsp90)
  19. Altmetric Badge
    Chapter 18 A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond
  20. Altmetric Badge
    Chapter 19 Computational Modeling of the Hsp90 Interactions with Cochaperones and Small-Molecule Inhibitors
  21. Altmetric Badge
    Chapter 20 Computational Analysis of the Chaperone Interaction Networks
  22. Altmetric Badge
    Chapter 21 Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer
  23. Altmetric Badge
    Chapter 22 Immunohistochemical and Flow Cytometric Analysis of Intracellular and Membrane-Bound Hsp70, as a Putative Biomarker of Glioblastoma Multiforme, Using the cmHsp70.1 Monoclonal Antibody
  24. Altmetric Badge
    Chapter 23 Detection and Analysis of Extracellular Hsp90 (eHsp90)
  25. Altmetric Badge
    Chapter 24 Molecular Chaperone Receptors
  26. Altmetric Badge
    Chapter 25 Creation of Recombinant Chaperone Vaccine Using Large Heat Shock Protein for Antigen-Targeted Cancer Immunotherapy
  27. Altmetric Badge
    Chapter 26 A Novel Heat Shock Protein 70-based Vaccine Prepared from DC-Tumor Fusion Cells
  28. Altmetric Badge
    Chapter 27 Hsp70: A Cancer Target Inside and Outside the Cell
  29. Altmetric Badge
    Chapter 28 Evidence for Hsp90 Co-chaperones in Regulating Hsp90 Function and Promoting Client Protein Folding
  30. Altmetric Badge
    Chapter 29 Clinical Evaluation and Biomarker Profiling of Hsp90 Inhibitors
Attention for Chapter 3: Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
1 tweeter

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
11 Mendeley

What is this page?

Summary Twitter Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Monitoring of the Heat Shock Response with a Real-Time Luciferase Reporter
Chapter number 3
Book title
Chaperones
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7477-1_3
Pubmed ID
29177649
Book ISBNs
978-1-4939-7476-4, 978-1-4939-7477-1
Authors

Toshiki Kijima, Takanori Eguchi, Len Neckers, Thomas L. Prince

Abstract

The heat shock response (HSR) is a cellular mechanism for counteracting acute proteotoxic stress. In eukaryotes, transcriptional activation of the HSR is regulated by heat shock factor 1 (HSF1). Activation of HSF1 induces the expression of heat shock proteins (HSPs) that function as molecular chaperones to fold and maintain the three-dimensional structure of misfolded proteins. The regulation of the degree and duration of the HSR is controlled by multiple biochemical mechanisms that include posttranslational modification of HSF1 and numerous protein-protein interactions. In this chapter, we describe a method to evaluate the activation and deactivation of the HSR at the transcriptional level using a short half-life luciferase reporter assay. This assay can be used to further characterize the HSR or as a screen for small-molecule inducers, amplifiers, or repressors.

View on publisher site

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 18%
Student > Bachelor 2 18%
Professor > Associate Professor 2 18%
Researcher 2 18%
Other 1 9%
Other 2 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 64%
Agricultural and Biological Sciences 1 9%
Medicine and Dentistry 1 9%
Unknown 2 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 December 2017.
All research outputs
#9,799,320
of 12,265,937 outputs
Outputs from Methods in molecular biology
#4,176
of 8,309 outputs
Outputs of similar age
#245,260
of 343,496 outputs
Outputs of similar age from Methods in molecular biology
#764
of 1,605 outputs
Altmetric has tracked 12,265,937 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,309 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 343,496 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 17th percentile – i.e., 17% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,605 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.