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Host-Pathogen Interactions

Overview of attention for book
Cover of 'Host-Pathogen Interactions'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Genetic Association Studies in Host–Pathogen Interaction Analysis
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    Chapter 2 Bacterial Genotyping Methods: From the Basics to Modern
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    Chapter 3 Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens
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    Chapter 4 Usage of a Bioluminescence Reporter System to Image Promoter Activity During Host Infection
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    Chapter 5 lacZ Reporter System as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens
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    Chapter 6 Western Blotting Against Tagged Virulence Determinants to Study Bacterial Pathogenicity
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    Chapter 7 Molecular Methods to Analyze the Effect of Proteins Expressed by Salmonella During Its Intracellular Stage
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    Chapter 8 Organoids as a Model to Study Infectious Disease
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    Chapter 9 Surface Proteome Biotinylation Combined with Bioinformatic Tools as a Strategy for Predicting Pathogen Interacting Proteins
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    Chapter 10 Systems Biology Modeling to Study Pathogen–Host Interactions
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    Chapter 11 Phage Therapy: Various Perspectives on How to Improve the Art
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    Chapter 12 Application of RNA-seq and Bioimaging Methods to Study Microbe–Microbe Interactions and Their Effects on Biofilm Formation and Gene Expression
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    Chapter 13 Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth
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    Chapter 14 Detection of Bacterial Quorum Sensing Molecules
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    Chapter 15 Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae
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    Chapter 16 Introduction of Genetic Material in Ralstonia solanacearum Through Natural Transformation and Conjugation
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    Chapter 17 In Vitro and In Vivo Secretion/Translocation Assays to Identify Novel Ralstonia solanacearum Type 3 Effectors
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    Chapter 18 Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains
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    Chapter 19 Methods to Quantify Biotic-Induced Stress in Plants
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    Chapter 20 From Sample to Data: Preparing, Obtaining, and Analyzing Images of Plant-Pathogen Interactions Using Confocal Microscopy
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    Chapter 21 Screening of c-di-GMP-Regulated Exopolysaccharides in Host Interacting Bacteria
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    Chapter 22 Primary Characterization of Small RNAs in Symbiotic Nitrogen-Fixing Bacteria
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    Chapter 23 A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean
  25. Altmetric Badge
    Chapter 24 Methods for the Characterization of Plant-Growth Promoting Rhizobacteria
Attention for Chapter 18: Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains
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Chapter title
Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains
Chapter number 18
Book title
Host-Pathogen Interactions
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7604-1_18
Pubmed ID
Book ISBNs
978-1-4939-7603-4, 978-1-4939-7604-1
Authors

Arry Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofei Jiang, Richard Berthomé, Alice Guidot

Abstract

In this chapter, we describe different methods for phenotyping strains or mutants of the bacterial wilt agent, Ralstonia solanacearum, on four different host plants: Arabidopsis thaliana, tomato (Solanum lycopersicum), tobacco (Nicotiana benthamiana), or Medicago truncatula. Methods for preparation of high volume or low volume inocula are first described. Then, we describe the procedures for inoculation of plants by soil drenching, stem injection or leaf infiltration, and scoring of the wilting symptoms development. Two methods for measurement of bacterial multiplication in planta are also proposed: (1) counting the bacterial colonies upon serial dilution plating and (2) determining the bacterial concentration using a qPCR approach. In this chapter, we also describe a competitive index assay to compare the fitness of two strains coinoculated in the same plant. Lastly, specific protocols describe in vitro and hydroponic inoculation procedures to follow disease development and bacterial multiplication in both the roots and aerial parts of the plant.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Student > Master 2 13%
Student > Bachelor 2 13%
Lecturer 1 7%
Librarian 1 7%
Other 1 7%
Unknown 4 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 47%
Biochemistry, Genetics and Molecular Biology 1 7%
Unspecified 1 7%
Arts and Humanities 1 7%
Chemistry 1 7%
Other 0 0%
Unknown 4 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 December 2017.
All research outputs
#11,840,372
of 13,345,024 outputs
Outputs from Methods in molecular biology
#5,981
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Outputs of similar age
#324,711
of 384,409 outputs
Outputs of similar age from Methods in molecular biology
#1,198
of 1,662 outputs
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