Chapter title |
Combining Cell Type-Restricted Adenoviral Targeting with Immunostaining and Flow Cytometry to Identify Cells-of-Origin of Lung Cancer
|
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Chapter number | 2 |
Book title |
Inflammation and Cancer
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7568-6_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7567-9, 978-1-4939-7568-6
|
Authors |
Sarah A. Best, Ariena Kersbergen, Marie-Liesse Asselin-Labat, Kate D. Sutherland, Best, Sarah A., Kersbergen, Ariena, Asselin-Labat, Marie-Liesse, Sutherland, Kate D. |
Abstract |
Lung cancers display considerable intertumoral heterogeneity, leading to the classification of distinct tumor subtypes. Our understanding of the genetic aberrations that underlie tumor subtypes has been greatly enhanced by recent genomic sequencing studies and state-of-the-art gene targeting technologies, highlighting evidence that distinct lung cancer subtypes may be derived from different "cells-of-origin". Here, we describe the intra-tracheal delivery of cell type-restricted Ad5-Cre viruses into the lungs of adult mice, combined with immunohistochemical and flow cytometry strategies for the detection of lung cancer-initiating cells in vivo. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 2 | 22% |
Librarian | 1 | 11% |
Other | 1 | 11% |
Student > Ph. D. Student | 1 | 11% |
Unknown | 4 | 44% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 2 | 22% |
Business, Management and Accounting | 1 | 11% |
Chemistry | 1 | 11% |
Agricultural and Biological Sciences | 1 | 11% |
Unknown | 4 | 44% |