| Chapter title |
Antimicrobial Peptides
|
|---|---|
| Chapter number | 15 |
| Book title |
Antimicrobial Peptides
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6737-7_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6735-3, 978-1-4939-6737-7
|
| Authors |
Nury P. Santisteban, Michael R. Morrow, Valerie Booth, Santisteban, Nury P., Morrow, Michael R., Booth, Valerie |
| Abstract |
Antimicrobial peptides (AMPs) may interact with a variety of target cell components, including the lipid bilayer, non-lipidic cell envelope components, and/or intracellular targets. However, most biophysical experiments aimed at elucidating the detailed mechanism of AMPs are limited to simple model membrane systems and neglect potentially functional interactions between AMPs and non-lipidic cell components. One of the biophysical techniques commonly used to study how AMPs interact with lipid bilayers is solid-state deuterium NMR. In this chapter we provide protocols to prepare deuterium-labeled intact Gram-negative and Gram-positive bacteria and to observe these samples using solid-state deuterium NMR. Such experiments have the potential to provide important information about how non-lipidic cell envelope components modulate AMP interactions with the cytoplasmic membrane of bacteria. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 25% |
| Student > Doctoral Student | 1 | 25% |
| Unknown | 2 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Physics and Astronomy | 1 | 25% |
| Unknown | 2 | 50% |