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Antimicrobial Peptides

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Cover of 'Antimicrobial Peptides'

Table of Contents

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    Book Overview
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    Chapter 1 Antimicrobial Peptides: An Introduction.
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    Chapter 2 Tools for Designing Amphipathic Helical Antimicrobial Peptides
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    Chapter 3 Chemical Synthesis of Antimicrobial Peptides
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    Chapter 4 Microwave-Assisted Synthesis of Antimicrobial Peptides
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    Chapter 5 High-Performance Liquid Chromatography and Mass Spectrometry-Based Design of Proteolytically Stable Antimicrobial Peptides
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    Chapter 6 Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR
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    Chapter 7 Mass Spectrometry Approaches for Determining the Structure of Antimicrobial Peptides
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    Chapter 8 Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptide–Lipid Bilayer Interactions
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    Chapter 9 Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides
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    Chapter 10 Fluorescence and Absorbance Spectroscopy Methods to Study Membrane Perturbations by Antimicrobial Host Defense Peptides
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    Chapter 11 Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption
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    Chapter 12 Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action
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    Chapter 13 Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides
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    Chapter 14 Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA
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    Chapter 15 Antimicrobial Peptides
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    Chapter 16 Preparation of Membrane Models of Gram-Negative Bacteria and Their Interaction with Antimicrobial Peptides Studied by CD and NMR
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    Chapter 17 Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells Using Circular Dichroism
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    Chapter 18 Studying the Mechanism of Membrane Permeabilization Induced by Antimicrobial Peptides Using Patch-Clamp Techniques
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    Chapter 19 Assays for Identifying Inducers of the Antimicrobial Peptide LL-37
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    Chapter 20 Methods for Elucidating the Mechanism of Action of Proline-Rich and Other Non-lytic Antimicrobial Peptides
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    Chapter 21 The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus aureus Investigated by ATP Leakage, DNA-Binding Analysis, and the Expression of a LexA-Controlled Gene, recA
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    Chapter 22 Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides
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    Chapter 23 Antimicrobial Peptides
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    Chapter 24 Protocols for Studying Antimicrobial Peptides (AMPs) as Anticancer Agents
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    Chapter 25 Antimicrobial Peptides
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    Chapter 26 Using Disease-Associated Enzymes to Activate Antimicrobial Peptide Prodrugs
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    Chapter 27 Anti-inflammatory Properties of Antimicrobial Peptides and Peptidomimetics: LPS and LTA Neutralization
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    Chapter 28 Protocols for Screening Antimicrobial Peptides That Influence Virulence Gene Expression in Staphylococcus aureus
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    Chapter 29 Methods for In Vitro Analysis of Antimicrobial Activity and Toxicity of Anti-keratitis Peptides: Bacterial Viability in Tears, MTT, and TNF-α Release Assays
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    Chapter 30 Methods for In Vivo/Ex Vivo Analysis of Antimicrobial Peptides in Bacterial Keratitis: siRNA Knockdown, Colony Counts, Myeloperoxidase, Immunostaining, and RT-PCR Assays
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    Chapter 31 Hemolytic Activity of Antimicrobial Peptides
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    Chapter 32 Erratum
Attention for Chapter 13: Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides
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Chapter title
Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides
Chapter number 13
Book title
Antimicrobial Peptides
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6737-7_13
Pubmed ID
Book ISBNs
978-1-4939-6735-3, 978-1-4939-6737-7
Authors

Gabriel Del Rio, Edda Klipp, Andreas Herrmann, Del Rio, Gabriel, Klipp, Edda, Herrmann, Andreas

Abstract

Antimicrobial peptides (AMPs) may display the ability to penetrate cells, which may be relevant for their antibiotic activity. To investigate the relevance of the penetrating activity for the antibiotic activity of AMPs, here we describe a method based on the combined use of confocal microscopy and computational modeling coupled with cell death kinetics.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 27%
Student > Master 2 18%
Other 1 9%
Lecturer 1 9%
Unknown 4 36%
Readers by discipline Count As %
Immunology and Microbiology 2 18%
Agricultural and Biological Sciences 1 9%
Biochemistry, Genetics and Molecular Biology 1 9%
Unknown 7 64%