Chapter title |
Methods for In Vivo/Ex Vivo Analysis of Antimicrobial Peptides in Bacterial Keratitis: siRNA Knockdown, Colony Counts, Myeloperoxidase, Immunostaining, and RT-PCR Assays
|
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Chapter number | 30 |
Book title |
Antimicrobial Peptides
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6737-7_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6735-3, 978-1-4939-6737-7
|
Authors |
Satya Sree Kolar, Hasna Baidouri, Maria Luisa Mangoni, Alison M. McDermott, Kolar, Satya Sree, Baidouri, Hasna, Mangoni, Maria Luisa, McDermott, Alison M. |
Abstract |
Antimicrobial peptides (AMPs) are essential components of the innate immune response. They have direct killing ability as well as immunomodulatory functions. Here, we describe techniques to identify specific AMPs involved in the protection against microbial keratitis, a vision threatening infection of the cornea of the eye which is the most serious complication of contact lens wear. Specifically we detail the use of siRNA technology to temporarily knockdown AMP expression at the murine ocular surface in vivo and then describe ex vivo assays to determine the level of bacteria, relative number of neutrophils, and levels of cytokines, chemokines, and AMPs in infected corneas. |
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Unknown | 1 | 25% |
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